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Acta Pharmacol Sin. 2016 Jan;37(1):34-43. doi: 10.1038/aps.2015.108. Epub 2015 Dec 14.

High throughput screening technologies for ion channels.

Author information

1
State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

Abstract

Ion channels are involved in a variety of fundamental physiological processes, and their malfunction causes numerous human diseases. Therefore, ion channels represent a class of attractive drug targets and a class of important off-targets for in vitro pharmacological profiling. In the past decades, the rapid progress in developing functional assays and instrumentation has enabled high throughput screening (HTS) campaigns on an expanding list of channel types. Chronologically, HTS methods for ion channels include the ligand binding assay, flux-based assay, fluorescence-based assay, and automated electrophysiological assay. In this review we summarize the current HTS technologies for different ion channel classes and their applications.

PMID:
26657056
PMCID:
PMC4722985
DOI:
10.1038/aps.2015.108
[Indexed for MEDLINE]
Free PMC Article

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