Format

Send to

Choose Destination
Stem Cell Reports. 2019 Jan 24. pii: S2213-6711(19)30007-4. doi: 10.1016/j.stemcr.2019.01.006. [Epub ahead of print]

Long-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesis.

Author information

1
Department of Pediatrics, Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
2
Center for Stem Cell Research, Institute of Molecular Medicine, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
3
Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
4
Center for Stem Cell Research, Institute of Molecular Medicine, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, TX 77030, USA. Electronic address: momoko.yoshimoto@uth.tmc.edu.

Abstract

It is generally considered that mouse embryonic stem cell (ESC) differentiation into blood cells in vitro recapitulates yolk sac (YS) hematopoiesis. As such, similar to YS-derived B-progenitors, we demonstrate here that ESC-derived B-progenitors differentiate into B-1 and marginal zone B cells, but not B-2 cells in immunodeficient mice after transplantation. ESC-derived B-1 cells were maintained in the recipients for more than 6 months, secreting natural IgM antibodies in vivo. Gene expression profiling displayed a close relationship between ESC- and YS-derived B-1 progenitors. Because there are no hematopoietic stem cells (HSCs) detectable in our ESC differentiation culture, successful long-term engraftment of ESC-derived functional B-1 cells supports the presence of HSC-independent B-1 cell development.

KEYWORDS:

B-1 cells; ESCs; HSC-independent lymphopoiesis; mouse embryo; yolk sac

PMID:
30745034
DOI:
10.1016/j.stemcr.2019.01.006
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center