Format

Send to

Choose Destination
Stem Cell Reports. 2019 Feb 12;12(2):201-212. doi: 10.1016/j.stemcr.2018.12.010. Epub 2019 Jan 10.

Astrocytes Regulate the Development and Maturation of Retinal Ganglion Cells Derived from Human Pluripotent Stem Cells.

Author information

1
Department of Biology, Indiana University-Purdue University Indianapolis, Indianapolis IN 46202, USA.
2
Department of Biology, Indiana University-Purdue University Indianapolis, Indianapolis IN 46202, USA; Stark Neurosciences Research Institute, Indiana University, Indianapolis IN 46202, USA.
3
Department of Biology, Indiana University-Purdue University Indianapolis, Indianapolis IN 46202, USA; Stark Neurosciences Research Institute, Indiana University, Indianapolis IN 46202, USA; Department of Medical and Molecular Genetics, Indiana University, Indianapolis IN 46202, USA; Glick Eye Institute, Department of Ophthalmology, Indiana University, Indianapolis IN 46202, USA. Electronic address: meyerjas@iupui.edu.

Abstract

Retinal ganglion cells (RGCs) form the connection between the eye and the brain, with this connectivity disrupted in numerous blinding disorders. Previous studies have demonstrated the ability to derive RGCs from human pluripotent stem cells (hPSCs); however, these cells exhibited some characteristics that indicated a limited state of maturation. Among the many factors known to influence RGC development in the retina, astrocytes are known to play a significant role in their functional maturation. Thus, efforts of the current study examined the functional maturation of hPSC-derived RGCs, including the ability of astrocytes to modulate this developmental timeline. Morphological and functional properties of RGCs were found to increase over time, with astrocytes significantly accelerating the functional maturation of hPSC-derived RGCs. The results of this study clearly demonstrate the functional and morphological maturation of RGCs in vitro, including the effects of astrocytes on the maturation of hPSC-derived RGCs.

KEYWORDS:

astrocyte; development; differentiation; pluripotent stem cell; retina; retinal ganglion cell; stem cell

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center