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Mater Today (Kidlington). 2018 Sep;21(7):703-712. doi: 10.1016/j.mattod.2018.03.002. Epub 2018 Apr 17.

Microfluidic generation of transient cell volume exchange for convectively driven intracellular delivery of large macromolecules.

Author information

1
Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
2
School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
3
Department of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
4
Department of Hematology and Medical Oncology, Emory University School of Medicine, Atlanta, Georgia, USA.

Abstract

Efficient intracellular delivery of target macromolecules remains a major obstacle in cell engineering and other biomedical applications. We discovered a unique cell biophysical phenomenon of transient cell volume exchange by using microfluidics to rapidly and repeatedly compress cells. This behavior consists of brief, mechanically induced cell volume loss followed by rapid volume recovery. We harness this behavior for high-throughput, convective intracellular delivery of large polysaccharides (2000 kDa), particles (100 nm), and plasmids while maintaining high cell viability. Successful proof of concept experiments in transfection and intracellular labeling demonstrated potential to overcome the most prohibitive challenges in intracellular delivery for cell engineering.

KEYWORDS:

Cell deformation; Cell engineering; Delivery; Microfluidics

PMID:
30288138
PMCID:
PMC6166476
[Available on 2019-09-01]
DOI:
10.1016/j.mattod.2018.03.002

Conflict of interest statement

Competing Financial Interests The authors declare no competing financial interests.

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