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J Nutr Biochem. 2019 May;67:36-43. doi: 10.1016/j.jnutbio.2019.01.021. Epub 2019 Feb 10.

Effect of annatto-extracted tocotrienols and green tea polyphenols on glucose homeostasis and skeletal muscle metabolism in obese male mice.

Author information

1
Department of Kinesiology, Health, and Nutrition, University of Texas at San Antonio, San Antonio, TX. Electronic address: Eunhee.chung@utsa.edu.
2
Department of Kinesiology, Health, and Nutrition, University of Texas at San Antonio, San Antonio, TX. Electronic address: salncampise@gmail.com.
3
Department of Kinesiology and Sport Management, Texas Tech University, Lubbock, TX. Electronic address: hej1704@hsutx.edu.
4
Department of Pathology, Texas Tech University Health Sciences Center, Lubbock, TX. Electronic address: michael.d.tomison@ttuhsc.edu.
5
Department of Medical Education, Texas Tech University Health Sciences Center, Lubbock, TX; Center of Excellence for Integrative Health, Texas Tech University Health Sciences Center, Lubbock, TX. Electronic address: Gurvinder.kaur@ttuhsc.edu.
6
Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX; Obesity Research Cluster, Texas Tech University, Lubbock, TX. Electronic address: Jannette.dufour@ttuhsc.edu.
7
Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX. Electronic address: Lillian.ene@ttuhsc.edu.
8
Center of Excellence for Integrative Health, Texas Tech University Health Sciences Center, Lubbock, TX; Obesity Research Cluster, Texas Tech University, Lubbock, TX; Department of Nutritional Sciences, Texas Tech University, Lubbock, TX. Electronic address: Latha.Ramalingam@ttu.edu.
9
Center of Excellence for Integrative Health, Texas Tech University Health Sciences Center, Lubbock, TX; Obesity Research Cluster, Texas Tech University, Lubbock, TX; Department of Nutritional Sciences, Texas Tech University, Lubbock, TX. Electronic address: naima.moustaid-moussa@ttu.edu.
10
Department of Pathology, Texas Tech University Health Sciences Center, Lubbock, TX; Center of Excellence for Integrative Health, Texas Tech University Health Sciences Center, Lubbock, TX; Obesity Research Cluster, Texas Tech University, Lubbock, TX. Electronic address: leslie.shen@ttuhsc.edu.

Abstract

Skeletal muscle is the major site for glucose uptake and thus plays an important role in initiating insulin resistance in type 2 diabetes mellitus. This study evaluated the effects of tocotrienols (TT) and green tea polyphenols (GTP) individually or in combination on glucose homeostasis and skeletal muscle metabolism in obese mice with insulin resistance and elevation of blood glucose. Forty-eight male mice were fed a high-fat diet and assigned to 4 groups in a 2 (no TT vs. 400 mg TT/kg diet) × 2 (no GTP vs. 0.5% vol/wt GTP in water) for 14 weeks. Both GTP and TT improved area under curve of insulin intolerance; while GTP increased serum insulin levels in obese mice, probably due to the addition of sweetener in drinking water. An interaction (TT×GTP) was observed in glucose tolerance test, total pancreas insulin concentration, and citrate synthase activity of soleus in mice. Neither TT nor GTP affected insulin and glucagon protein expression in pancreas based on immunohistochemistry. Both TT and GTP individually increased soleus muscle weight of mice; while only GTP increased gastrocnemius muscle weight of mice. The TT+GTP group had the greatest gastrocnemius muscle cross sectional area than other groups. GTP, not TT, induced cytochrome c oxidase activity and reduced thiobarbituric acid reactive substances levels in soleus muscle. Our results suggest that TT and GTP, individually or synergistically have the potential to improve skeletal muscle metabolism in obese mice by improving glucose homeostasis, reducing lipid peroxidation, and increasing rate limiting enzymes of oxidative phosphorylation.

KEYWORDS:

Aging; Animals; Diabetes; Dietary supplement; Obesity; Sarcopenia

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