Format

Send to

Choose Destination
Biosens Bioelectron. 2019 Apr 15;131:46-52. doi: 10.1016/j.bios.2019.01.040. Epub 2019 Jan 29.

An ultra-sensitive capacitive microwire sensor for pathogen-specific serum antibody responses.

Author information

1
School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA.
2
Cell and Molecular Biology Program, Colorado State University, Fort Collins, CO 80523, USA; Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
3
Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
4
School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA; Department of Chemistry, Colorado State University, Fort Collins, CO 80523, USA.
5
School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA; Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO 80523, USA. Electronic address: David.Dandy@colostate.edu.
6
School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA; Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA. Electronic address: Brian.Geiss@colostate.edu.

Abstract

Detection of viral infection is commonly performed using serological techniques like the enzyme-linked immunosorbent assay (ELISA) to detect antibody responses. Such assays may also be used to determine the infection phase based on isotype prevalence. However, ELISAs demonstrate limited sensitivity and are difficult to perform at the point of care. Here, we present a novel technique for label-free, rapid detection of ultra-low concentrations of virus specific antibodies. We have developed a simple, robust capacitive biosensor using microwires coated with Zika or Chikungunya virus envelope antigen. With little discernable nonspecific binding, the sensor can detect as few as 10 antibody molecules in a small volume (10 molecules/30 µL) within minutes. It can also be used to rapidly, specifically, and accurately determine the isotype of antigen-specific antibodies. Finally, we demonstrate that anti-Zika virus antibody can be sensitively and specifically detected in dilute mouse serum and can be isotyped using the sensor. Overall, our findings suggest that our microwire sensor platform has the potential to be used as a reliable, sensitive, and inexpensive diagnostic tool to detect immune responses at the point of care.

KEYWORDS:

Antibody detection; Capacitive affinity biosensor; Isotype; Microwires; Mouse serum; Zika virus

PMID:
30822687
PMCID:
PMC6422737
[Available on 2020-04-15]
DOI:
10.1016/j.bios.2019.01.040

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center