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Mol Biochem Parasitol. 2018 Oct;225:84-93. doi: 10.1016/j.molbiopara.2018.09.003. Epub 2018 Sep 21.

TbUTP10, a protein involved in early stages of pre-18S rRNA processing in Trypanosoma brucei.

Author information

1
Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005, České Budějovice (Budweis), Czech Republic; Faculty of Sciences, University of South Bohemia, 37005, České Budějovice, Czech Republic. Electronic address: dranov@paru.cas.cz.
2
Faculty of Sciences, University of South Bohemia, 37005, České Budějovice, Czech Republic.
3
Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005, České Budějovice (Budweis), Czech Republic; Faculty of Sciences, University of South Bohemia, 37005, České Budějovice, Czech Republic.
4
Department of Microbiology, The Ohio State University, Columbus, OH, USA; The Center for RNA Biology, The Ohio State University, Columbus, OH, USA.
5
Centre of Immunity, Infection and Evolution and Institute of Immunology & Infection Research, University of Edinburgh, Edinburgh, United Kingdom.
6
Department of Microbiology, The Ohio State University, Columbus, OH, USA; The Center for RNA Biology, The Ohio State University, Columbus, OH, USA; The Ohio State Biochemistry Program, The Ohio State University, Columbus, OH, USA.

Abstract

Ribosome biosynthesis, best studied in opisthokonts, is a highly complex process involving numerous protein and RNA factors. Yet, very little is known about the early stages of pre-18S rRNA processing even in these model organisms, let alone the conservation of this mechanism in other eukaryotes. Here we extend our knowledge of this process by identifying and characterizing the essential protein TbUTP10, a homolog of yeast U3 small nucleolar RNA-associated protein 10 - UTP10 (HEATR1 in human), in the excavate parasitic protist Trypanosoma brucei. We show that TbUTP10 localizes to the nucleolus and that its ablation by RNAi knock-down in two different T. brucei life cycle stages results in similar phenotypes: a disruption of pre-18S rRNA processing, exemplified by the accumulation of rRNA precursors, a reduction of mature 18S rRNA, and also a decrease in the level of U3 snoRNA. Moreover, polysome profiles of the RNAi-induced knock-down cells show a complete disappearance of the 40S ribosomal subunit, and a prominent accumulation of the 60S large ribosomal subunit, reflecting impaired ribosome assembly. Thus, TbUTP10 is an important protein in the processing of 18S rRNA.

KEYWORDS:

Pre-18S rRNA processing; Ribosomal RNA; Trypanosoma; U3 snoRNA; UTP10

PMID:
30248370
PMCID:
PMC6556232
DOI:
10.1016/j.molbiopara.2018.09.003
[Indexed for MEDLINE]
Free PMC Article

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