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Int J Parasitol. 2018 Jun;48(7):519-530. doi: 10.1016/j.ijpara.2017.11.008. Epub 2018 Mar 10.

Diversity of Entamoeba spp. in African great apes and humans: an insight from Illumina MiSeq high-throughput sequencing.

Author information

1
Department of Pathology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1946/1, Brno 61242, Czech Republic; Institute of Vertebrate Biology, Czech Academy of Sciences, Květná 8, Brno 603 65, Czech Republic. Electronic address: klari.vlckova@gmail.com.
2
Division of Animal Evolutionary Biology, Department of Zoology Faculty of Science, Charles University in Prague, Viničná 7, 128 44 Praha, Czech Republic.
3
Department of Pathology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1946/1, Brno 61242, Czech Republic.
4
Institute of Vertebrate Biology, Czech Academy of Sciences, Květná 8, Brno 603 65, Czech Republic.
5
Projet Grands Singes Cameroon, Centre for Research and Conservation, Royal Zoological Society of Antwerp, 20-26 Koningin Astridplein, 2018 Antwerp, Belgium.
6
Institute of Parasitology, University of Zurich, Winterthurerstrasse 266a, CH-8057 Zrich, Switzerland.
7
Department of Pathology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1946/1, Brno 61242, Czech Republic; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, České Budějovice 370 05, Czech Republic; CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1946/1, Brno 612 42, Czech Republic.

Abstract

Understanding the complex Entamoeba communities in the mammalian intestine has been, to date, complicated by the lack of a suitable approach for molecular detection of multiple variants co-occurring in mixed infections. Here, we report on the application of a high throughput sequencing approach based on partial 18S rDNA using the Illumina MiSeq platform. We describe, to our knowledge, for the first time, the Entamoeba communities in humans, free-ranging western lowland gorillas and central chimpanzees living in the Dja Faunal Reserve in Cameroon. We detected 36 Entamoeba haplotypes belonging to six haplotype clusters, containing haplotypes possessing high and low host specificity. Most of the detected haplotypes belonged to commensal Entamoeba, however, the pathogenic species (Entamoeba histolytica and Entamoeba nuttalli) were also detected. We observed that some Entamoeba haplotypes are shared between humans and other hosts, indicating their zoonotic potential. The findings are important not only for understanding the epidemiology of amoebiasis in humans in rural African localities, but also in the context of wild great ape conservation.

KEYWORDS:

Central chimpanzee; Diversity; Entamoeba; Entamoeba histolytica; Humans; Metabarcoding; Mixed infections; Western lowland gorilla

PMID:
29530647
DOI:
10.1016/j.ijpara.2017.11.008
[Indexed for MEDLINE]

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