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Environ Toxicol Pharmacol. 2013 Sep;36(2):243-255. doi: 10.1016/j.etap.2013.04.007. Epub 2013 Apr 25.

β-N-methylamino-l-alanine causes neurological and pathological phenotypes mimicking Amyotrophic Lateral Sclerosis (ALS): the first step towards an experimental model for sporadic ALS.

Author information

1
Departamento de Biología Animal II, Universidad Complutense de Madrid, 28040 Madrid, Spain.
2
Departamento de Bioquímica y Biología Molecular I, Universidad Complutense de Madrid, 28040 Madrid, Spain.
3
Departamento de Biología Celular, Universidad Complutense de Madrid, 28040 Madrid, Spain.
4
Instituto de Química Médica - Centro Superior de Investigaciones Científicas, 28006 Madrid, Spain.
5
Departamento de Biología Animal II, Universidad Complutense de Madrid, 28040 Madrid, Spain. Electronic address: rportero@bio.ucm.es.

Abstract

β-N-methylamino-l-alanine (L-BMAA) is a neurotoxic amino acid that has been related to various neurodegenerative diseases. The aim of this work was to analyze the biotoxicity produced by L-BMAA in vivo in rats, trying to elucidate its physiopathological mechanisms and to search for analogies between the found effects and pathologies like Amyotrophic Lateral Sclerosis (ALS). Our data demonstrated that the neurotoxic effects in vivo were dosage-dependent. For evaluating the state of the animals, a neurological evaluation scale was developed as well as a set of functional tests. Ultrastructural cell analysis of spinal motoneurons has revealed alterations both in endoplasmic reticulum and mitochondria. Since GSK3β could play a role in some neuropathological processes, we analyzed the alterations occurring in GSK3β levels in L-BMAA treated rats, we have observed an increase in the active form of GSK3β levels in lumbar spinal cord and motor cerebral cortex. On the other hand, (TAR)-DNA-binding protein 43 (TDP-43) increased in L-BMAA treated animals. Our results indicated that N-acetylaspartate (NAA) declined in animals treated with L-BMAA, and the ratio of N-acetylaspartate/choline (NAA/Cho), N-acetylaspartate/creatine (NAA/Cr) and N-acetylaspartate/choline+creatine (NAA/Cho+Cr) tended to decrease in lumbar spinal cord and motor cortex. This project offers some encouraging results that could help establishing the progress in the development of an animal model of sporadic ALS and L-BMAA could be a useful tool for this purpose.

KEYWORDS:

(TAR)-DNA-binding protein 43; ALS; ALS-PDC; Amyotrophic Lateral Sclerosis; Amyotrophic Lateral Sclerosis/Parkinson-Dementia complex; Cho; Cr; ER; GSK3β; L-BMAA; Lumbar spinal cord; Motor cortex; N-acetylaspartate; NAA; Rat sporadic Amyotrophic Lateral Sclerosis model; TDP-43; choline; creatine; endoplasmic reticulum; glycogen synthase kinase-3β; β-N-methylamino-l-alanine

PMID:
23688553
DOI:
10.1016/j.etap.2013.04.007
[Indexed for MEDLINE]

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