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Methods Mol Biol. 2013;959:265-75. doi: 10.1007/978-1-62703-221-6_18.

Regeneration in Arabidopsis tissue culture.

Author information

1
Division of Biology 156-29, California Institute of Technology, Pasadena, CA, USA.

Abstract

An entire Arabidopsis plant can be regenerated from a small piece of tissue by two sequential hormonal treatments in tissue culture. Currently this in vitro regeneration system is a good system to study the mechanism by which plants show regenerative plasticity. Also, it is useful to test the hormone sensitivity of plants and to propagate sterile lines in Arabidopsis. Here we describe a standard protocol for regenerating Arabidopsis plants in tissue culture, and for preparing and observing samples using confocal microscopy to study cells during regeneration.

PMID:
23299682
DOI:
10.1007/978-1-62703-221-6_18
[Indexed for MEDLINE]

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