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Bioessays. 2016 Jul;38(7):627-43. doi: 10.1002/bies.201600057. Epub 2016 May 27.

Bistability of mitotic entry and exit switches during open mitosis in mammalian cells.

Author information

1
Genome Damage and Stability Centre, University of Sussex, Brighton, UK.
2
Department of Biochemistry, Oxford Centre for Integrative Systems Biology, University of Oxford, Oxford, UK.

Abstract

Mitotic entry and exit are switch-like transitions that are driven by the activation and inactivation of Cdk1 and mitotic cyclins. This simple on/off reaction turns out to be a complex interplay of various reversible reactions, feedback loops, and thresholds that involve both the direct regulators of Cdk1 and its counteracting phosphatases. In this review, we summarize the interplay of the major components of the system and discuss how they work together to generate robustness, bistability, and irreversibility. We propose that it may be beneficial to regard the entry and exit reactions as two separate reversible switches that are distinguished by differences in the state of phosphatase activity, mitotic proteolysis, and a dramatic rearrangement of cellular components after nuclear envelope breakdown, and discuss how the major Cdk1 activity thresholds could be determined for these transitions.

KEYWORDS:

Cdc25; Greatwall; MPF; Wee1; mammalian cells; mitosis

PMID:
27231150
DOI:
10.1002/bies.201600057
[Indexed for MEDLINE]

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