Single-molecule visualization of the hybridization and dissociation of photoresponsive oligonucleotides and their reversible switching behavior in a DNA nanostructure

Masayuki Endo; Yangyang Yang; Yuki Suzuki; Kumi Hidaka; Hiroshi Sugiyama

doi:10.1002/anie.201205247

Single-molecule visualization of the hybridization and dissociation of photoresponsive oligonucleotides and their reversible switching behavior in a DNA nanostructure

Angew Chem Int Ed Engl. 2012 Oct 15;51(42):10518-22. doi: 10.1002/anie.201205247. Epub 2012 Sep 10.

Authors

Masayuki Endo¹, Yangyang Yang, Yuki Suzuki, Kumi Hidaka, Hiroshi Sugiyama

Affiliation

¹ Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501, Japan. endo@kuchem.kyoto-u.ac.jp

PMID: 22965475
DOI: 10.1002/anie.201205247

Abstract

A framed photo of DNA: A pair of photoresponsive oligonucleotides containing azobenzene moieties was introduced into double-stranded DNA within the cavity of a DNA nanostructure (see scheme). The two dsDNAs, in contact at the center, were dissociated using UV irradiation and hybridized with visible light; this was directly observed using high-speed atomic force microscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemistry*
Nanostructures / chemistry*
Nanotechnology / methods
Nucleic Acid Hybridization / methods
Oligonucleotides / chemistry*
Photochemistry

Substances

Oligonucleotides
DNA