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Methods Mol Biol. 2016;1458:137-57. doi: 10.1007/978-1-4939-3801-8_11.

Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes.

Author information

1
Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, McMaster University, 1200 Main Street West, Hamilton, ON, Canada, L8N 3Z5.
2
Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, McMaster University, 1200 Main Street West, Hamilton, ON, Canada, L8N 3Z5. bramsonj@mcmaster.ca.

Abstract

The adoptive transfer of a bolus of tumor-specific T lymphocytes into cancer patients is a promising therapeutic strategy. In one approach, tumor specificity is conferred upon T cells via engineering expression of exogenous receptors, such as chimeric antigen receptors (CARs). Here, we describe the generation and production of both murine and human CAR-engineered T lymphocytes using retroviruses.

KEYWORDS:

CAR-T cell; Chimeric antigen receptor; Gamma-retrovirus; Lentivirus; T lymphocytes

PMID:
27581020
DOI:
10.1007/978-1-4939-3801-8_11
[Indexed for MEDLINE]

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