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Adv Mater. 2016 Mar 9;28(10):2024-9. doi: 10.1002/adma.201504154. Epub 2015 Dec 21.

Multiprotein Printing by Light-Induced Molecular Adsorption.

Author information

1
Interdisciplinary Institute for Neuroscience, University of Bordeaux, F-33077, Bordeaux, France.
2
CNRS UMR 5297, F-33077, Bordeaux, France.
3
Ecole Nationale Supérieure de Biotechnologie, Université Ali Mendjeli, BP E66, 25100, Constantine, Algeria.

Abstract

Light-induced molecular adsorption of proteins (LIMAP) allows for quantitative sub-micrometer-resolution printing of multiple biomolecules. Surface-bound gradients are patterned within minutes over an entire glass cover-slip. LIMAP is used to perform selective immuno-assays, to dynamically control the adhesion of individual cells, and to achieve hierarchical co-cultures instrumental for tissue engineering.

KEYWORDS:

cell co-culture; photopatterning; poly(ethylene glycol); protein micropatterning; single molecules

PMID:
26689426
DOI:
10.1002/adma.201504154
[Indexed for MEDLINE]

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