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J Vis Exp. 2008 Jan 16;(11). pii: 634. doi: 10.3791/634.

Murine skin transplantation.

Author information

1
Department of Physiology and Biophysics, University of California, Irvine, USA.

Abstract

As one of the most stringent and least technically challenging models, skin transplantation is a standard method to assay host T cell responses to MHC-disparate donor antigens. The aim of this video-article is to provide the viewer with a step-by-step visual demonstration of skin transplantation using the mouse model. The protocol is divided into 5 main components: 1) harvesting donor skin; 2) preparing recipient for transplant; 3) skin transplant; 4) bandage removal and monitoring graft rejection; 5) helpful hints. Once proficient, the procedure itself should take <10 min to perform.

PMID:
19066559
PMCID:
PMC2582837
DOI:
10.3791/634
[Indexed for MEDLINE]
Free PMC Article

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