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Generation of human induced pluripotent stem cell-derived cardiomyocytes in 2D monolayer and scalable 3D suspension bioreactor cultures with reduced batch-to-batch variations.

Hamad S, Derichsweiler D, Papadopoulos S, Nguemo F, Šarić T, Sachinidis A, Brockmeier K, Hescheler J, Boukens BJ, Pfannkuche K.

Theranostics. 2019 Sep 25;9(24):7222-7238. doi: 10.7150/thno.32058. eCollection 2019.


In Vitro Grown Micro-Tissues for Cardiac Cell Replacement Therapy in Vivo.

Sahito RGA, Sheng X, Maass M, Mikhael N, Hamad S, Heras-Bautista CO, Derichsweiler D, Spitkovsky D, Suhr F, Khalil M, Brockmeier K, Halbach M, Saric T, Hescheler J, Krausgrill B, Pfannkuche K.

Cell Physiol Biochem. 2019;52(6):1309-1324. doi: 10.33594/000000092.


Cardiomyocytes facing fibrotic conditions re-express extracellular matrix transcripts.

Heras-Bautista CO, Mikhael N, Lam J, Shinde V, Katsen-Globa A, Dieluweit S, Molcanyi M, Uvarov V, Jütten P, Sahito RGA, Mederos-Henry F, Piechot A, Brockmeier K, Hescheler J, Sachinidis A, Pfannkuche K.

Acta Biomater. 2019 Apr 15;89:180-192. doi: 10.1016/j.actbio.2019.03.017. Epub 2019 Mar 9.


The Potential Application of Biomaterials in Cardiac Stem Cell Therapy.

Sahito RG, Sureshkumar P, Sotiriadou I, Srinivasan SP, Sabour D, Hescheler J, Pfannkuche K, Sachinidis A.

Curr Med Chem. 2016;23(6):589-602. Review.


The Interaction between Adult Cardiac Fibroblasts and Embryonic Stem Cell-Derived Cardiomyocytes Leads to Proarrhythmic Changes in In Vitro Cocultures.

Trieschmann J, Bettin D, Haustein M, Köster A, Molcanyi M, Halbach M, Hanna M, Fouad M, Brockmeier K, Hescheler J, Pfannkuche K, Hannes T.

Stem Cells Int. 2016;2016:2936126. doi: 10.1155/2016/2936126. Epub 2016 Jan 5.


Preface. Cell fusion.

Pfannkuche K.

Methods Mol Biol. 2015;1313:v. No abstract available.


Electrophysiological characteristics of embryonic stem cell-derived cardiomyocytes are cell line-dependent.

Hannes T, Wolff M, Doss MX, Pfannkuche K, Haustein M, Müller-Ehmsen J, Sachinidis A, Hescheler J, Khalil M, Halbach M.

Cell Physiol Biochem. 2015;35(1):305-14. doi: 10.1159/000369697. Epub 2015 Jan 10.


Functional expression and regulation of hyperpolarization-activated cyclic nucleotide-gated channels (HCN) in mouse iPS cell-derived cardiomyocytes after UTF1 -neo selection.

Semmler J, Lehmann M, Pfannkuche K, Reppel M, Hescheler J, Nguemo F.

Cell Physiol Biochem. 2014;34(4):1199-215. doi: 10.1159/000366332. Epub 2014 Sep 25.


The influence of physiological matrix conditions on permanent culture of induced pluripotent stem cell-derived cardiomyocytes.

Heras-Bautista CO, Katsen-Globa A, Schloerer NE, Dieluweit S, Abd El Aziz OM, Peinkofer G, Attia WA, Khalil M, Brockmeier K, Hescheler J, Pfannkuche K.

Biomaterials. 2014 Aug;35(26):7374-85. doi: 10.1016/j.biomaterials.2014.05.027. Epub 2014 Jun 2.


Mesenchymal stem cells and their conditioned medium improve integration of purified induced pluripotent stem cell-derived cardiomyocyte clusters into myocardial tissue.

Rubach M, Adelmann R, Haustein M, Drey F, Pfannkuche K, Xiao B, Koester A, Udink ten Cate FE, Choi YH, Neef K, Fatima A, Hannes T, Pillekamp F, Hescheler J, Šarić T, Brockmeier K, Khalil M.

Stem Cells Dev. 2014 Mar 15;23(6):643-53. doi: 10.1089/scd.2013.0272. Epub 2014 Jan 15.


Evidence for self-maintaining pluripotent murine stem cells in embryoid bodies.

Attia WA, Abd El Aziz OM, Spitkovsky D, Gaspar JA, Dröge P, Suhr F, Sabour D, Winkler J, Meganathan K, Jagtap S, Khalil M, Hescheler J, Konrad Brockmeier, Agapios Sachinidis, Kurt Pfannkuche.

Stem Cell Rev Rep. 2014 Feb;10(1):1-15.


Evidence for a critical role of catecholamines for cardiomyocyte lineage commitment in murine embryonic stem cells.

Lehmann M, Nguemo F, Wagh V, Pfannkuche K, Hescheler J, Reppel M.

PLoS One. 2013 Aug 2;8(8):e70913. doi: 10.1371/journal.pone.0070913. Print 2013.


Cell biological effects of mechanical stimulations generated by focused extracorporeal shock wave applications on cultured human bone marrow stromal cells.

Suhr F, Delhasse Y, Bungartz G, Schmidt A, Pfannkuche K, Bloch W.

Stem Cell Res. 2013 Sep;11(2):951-64. doi: 10.1016/j.scr.2013.05.010. Epub 2013 May 29.


The L-type Ca2+ channels blocker nifedipine represses mesodermal fate determination in murine embryonic stem cells.

Nguemo F, Fleischmann BK, Gupta MK, Sarić T, Malan D, Liang H, Pfannkuche K, Bloch W, Schunkert H, Hescheler J, Reppel M.

PLoS One. 2013;8(1):e53407. doi: 10.1371/journal.pone.0053407. Epub 2013 Jan 8.


Intense resistance exercise induces early and transient increases in ryanodine receptor 1 phosphorylation in human skeletal muscle.

Gehlert S, Bungartz G, Willkomm L, Korkmaz Y, Pfannkuche K, Schiffer T, Bloch W, Suhr F.

PLoS One. 2012;7(11):e49326. doi: 10.1371/journal.pone.0049326. Epub 2012 Nov 16.


Human pluripotent stem cell-derived cardiomyocytes: response to TTX and lidocain reveals strong cell to cell variability.

Sheng X, Reppel M, Nguemo F, Mohammad FI, Kuzmenkin A, Hescheler J, Pfannkuche K.

PLoS One. 2012;7(9):e45963. doi: 10.1371/journal.pone.0045963. Epub 2012 Sep 27.


Time-course of the electrophysiological maturation and integration of transplanted cardiomyocytes.

Halbach M, Krausgrill B, Hannes T, Wiedey M, Peinkofer G, Baumgartner S, Sahito RG, Pfannkuche K, Pillekamp F, Reppel M, Müller-Ehmsen J, Hescheler J.

J Mol Cell Cardiol. 2012 Sep;53(3):401-8. doi: 10.1016/j.yjmcc.2012.06.007. Epub 2012 Jun 21.


In vitro model for assessing arrhythmogenic properties of drugs based on high-resolution impedance measurements.

Nguemo F, Šarić T, Pfannkuche K, Watzele M, Reppel M, Hescheler J.

Cell Physiol Biochem. 2012;29(5-6):819-32. doi: 10.1159/000188069. Epub 2012 May 11.


Contractile properties of early human embryonic stem cell-derived cardiomyocytes: beta-adrenergic stimulation induces positive chronotropy and lusitropy but not inotropy.

Pillekamp F, Haustein M, Khalil M, Emmelheinz M, Nazzal R, Adelmann R, Nguemo F, Rubenchyk O, Pfannkuche K, Matzkies M, Reppel M, Bloch W, Brockmeier K, Hescheler J.

Stem Cells Dev. 2012 Aug 10;21(12):2111-21. doi: 10.1089/scd.2011.0312. Epub 2012 Mar 14.


Derivation of es Cells From Early Stage Preimplantation Embryos and Characterisation of their Cardiac Differentiation Potential in Mice.

Bag S, Pfannkuche K, Krzyzak V, Wagner B, Sachinidis A, Hescheler J.

J Stem Cells Regen Med. 2012 Apr 14;8(1):12-20. eCollection 2012.

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