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Transl Oncol. 2016 Apr;9(2):124-129. doi: 10.1016/j.tranon.2016.02.001.

Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems.

Author information

1
Department of Neurosurgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; Hugo W. Moser Research Institute at Kennedy Krieger, Baltimore, MD, USA. Electronic address: rory@jhmi.edu.
2
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
3
Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; Hugo W. Moser Research Institute at Kennedy Krieger, Baltimore, MD, USA.
4
Department of Ophthalmology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
5
Department of Neurosurgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
6
Hugo W. Moser Research Institute at Kennedy Krieger, Baltimore, MD, USA.

Abstract

Protein microarray technology has been successfully used for identifying substrates of purified activated kinases. We used protein microarrays to globally interrogate the effects of PTEN and Akt activity on the phospho-kinome of in vitro and in vivo glioma models and validated results in clinical pathological specimens. Whole cell lysates extracted from tumor samples can be applied to human kinome chip microarrays to profile the global kinase phosphorylation patterns in a high-throughput manner and identify novel substrates inherent to the tumor cell and the interactions with tumor microenvironment. Our findings identify a novel microarray-based method for assessing intracellular signaling events applicable to human oncogenesis and other pathophysiologic states.

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