Abstract
To identify factors that regulate proliferin (PLF) and PLF-related protein (PRP) secretion by the mouse placenta, placental cells from day 9 of pregnancy were cultured for up to 5 days, and PLF and PRP release into the medium was assessed by RIA. Transforming growth factor-alpha, interleukin-1 alpha, and interleukin-6 did not regulate either PLF or PRP secretion. However, treatment of primary placental cell cultures with 8-bromo-cAMP, cholera toxin, or forskolin resulted in 2- to 3-fold increases in the percentages of PLF- and PRP-producing cells in the population and corresponding increases in both PLF and PRP messenger RNA and secreted protein. The increase in the number of PLF-producing cells was accompanied by an increase in the number of cells expressing both PLF and mouse placental lactogen-I. These data suggest that cAMP levels can regulate trophoblast giant cell differentiation and, consequently, the amount of PLF and PRP secretion.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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8-Bromo Cyclic Adenosine Monophosphate / pharmacology
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Animals
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Cells, Cultured
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Cholera Toxin / pharmacology
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Colforsin / pharmacology
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Cyclic AMP / metabolism*
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Female
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Glycoproteins / biosynthesis*
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Glycoproteins / metabolism
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Growth Substances / biosynthesis*
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Intercellular Signaling Peptides and Proteins
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Interleukin-1 / pharmacology
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Interleukin-5 / pharmacology
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Kinetics
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Mice
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Placenta / cytology
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Placenta / drug effects
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Placenta / metabolism*
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Pregnancy
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Pregnancy Proteins / biosynthesis*
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Pregnancy Proteins / metabolism
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Prolactin
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Radioimmunoassay
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Time Factors
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Transforming Growth Factor alpha / pharmacology
Substances
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Glycoproteins
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Growth Substances
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Intercellular Signaling Peptides and Proteins
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Interleukin-1
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Interleukin-5
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Plfr protein, mouse
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Pregnancy Proteins
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Prl2c2 protein, mouse
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Transforming Growth Factor alpha
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Colforsin
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8-Bromo Cyclic Adenosine Monophosphate
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Prolactin
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Cholera Toxin
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Cyclic AMP