Labelling HaloTag Fusion Proteins with HaloTag Ligand in Living Cells

Bio Protoc. 2017 Sep 5;7(17):e2526. doi: 10.21769/BioProtoc.2526.

Abstract

HaloTag has been widely used to label proteins in vitro and in vivo (Los et al., 2008). In this protocol, we describe labelling HaloTag-Cbx fusion proteins by HaloTag ligands for live-cell single-molecule imaging (Zhen et al., 2016).

Keywords: Cbx; Epigenetics; HaloTag; Janelia Fluorâ„¢ dye; Live-cell single-molecule imaging; Polycomb.