HaloTag has been widely used to label proteins in vitro and in vivo (Los et al., 2008). In this protocol, we describe labelling HaloTag-Cbx fusion proteins by HaloTag ligands for live-cell single-molecule imaging (Zhen et al., 2016).
Keywords: Cbx; Epigenetics; HaloTag; Janelia Fluorâ„¢ dye; Live-cell single-molecule imaging; Polycomb.