Isolation of dense core secretory vesicles from pancreatic endocrine cells by differential and density gradient centrifugation

John C Hutton; Randall Wong; Howard W Davidson

doi:10.1002/0471143030.cb0332s42

Isolation of dense core secretory vesicles from pancreatic endocrine cells by differential and density gradient centrifugation

Curr Protoc Cell Biol. 2009 Mar:Chapter 3:Unit 3.32. doi: 10.1002/0471143030.cb0332s42.

Authors

John C Hutton¹, Randall Wong, Howard W Davidson

Affiliation

¹ Barbara Davis Center for Childhood Diabetes, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado, USA.

PMID: 19283733
DOI: 10.1002/0471143030.cb0332s42

Abstract

Methods are presented for the separation of dense core secretory vesicles from insulin-secreting tissues (insulin granules) based on a combination of differential and density gradient centrifugation on various media. Emphasis is given to the use of transplantable tumors, tissue culture cell lines, and pancreatic islets as a tissue source.

MeSH terms

Animals
Cell Fractionation / methods*
Cell Line
Centrifugation, Density Gradient
Insulin-Secreting Cells / ultrastructure*
Insulinoma / ultrastructure
Iohexol
Islets of Langerhans / ultrastructure
Neoplasms, Experimental / pathology
Pancreatic Neoplasms / ultrastructure
Povidone
Rats
Secretory Vesicles*
Silicon Dioxide

Substances

Iohexol
Percoll
Silicon Dioxide
Povidone