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J Integr Plant Biol. 2018 Aug;60(8):691-702. doi: 10.1111/jipb.12658. Epub 2018 Jul 4.

bHLH104 confers tolerance to cadmium stress in Arabidopsis thaliana.

Yao X1,2, Cai Y1,2, Yu D1, Liang G1.

Author information

1
Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China.
2
University of Chinese Academy of Sciences, Beijing 100049, China.

Abstract

Cd is a non-essential heavy metal that is toxic to both plants and animals. Here, we reveal that the transcription factor bHLH104 positively regulates Cd tolerance in Arabidopsis thaliana. We show that Fe deficiency-responsive genes were induced by Cd treatment, and that their upregulation was suppressed in bhlh104 loss-of-function mutants, but enhanced upon overexpression of bHLH104. Correspondingly, the bhlh104 mutants displayed sensitivity to Cd stress, whereas plants overexpressing bHLH104 exhibited enhanced Cd tolerance. Further analysis suggested that bHLH104 positively regulates four heavy metal detoxification-associated genes, IREG2, MTP3, HMA3 and NAS4, which play roles in Cd sequestration and tolerance. The bHLH104 overexpression plants accumulated high levels of Cd in the root but low levels of Cd in the shoot, which might contribute to the Cd tolerance in those lines. The present study thus points to bHLH104 as a potentially useful tool for genetic engineering of plants with enhanced Cd tolerance.

PMID:
29667322
DOI:
10.1111/jipb.12658
[Indexed for MEDLINE]

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