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Zhongguo Zhong Yao Za Zhi. 2016 May;41(9):1635-1639. doi: 10.4268/cjcmm20160911.

[Evaluation for extraction process of Shenqi Tongmai Yizhi particles based on antioxidant capacity in vitro and its spectrum-effect relation].

[Article in Chinese]

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Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
School of Chinese Materia Medica, Jilin Agricultural University, Changchun 130118, China.


Based on DPPH method, the antioxidant activities of Shenqi Tongmai Yizhi particles with different extraction processes were compared. The contribution to the anti-oxidant capacity in vitro was explored by means of grey relational analysis on different chemical compositions in the fingerprint. The results showed that the IC₅₀ concentration values of water extract, water extract from alcohol precipitation, alcohol extract, and alcohol and water extract were 0.801 4, 0.859 1, 0.796 1, 0.918 0 g•L⁻¹; and the alcohol extract is the best method to extract antioxidative components, with the highest antioxidant activity and lowest IC₅₀. When the mass concentration of the herbs reached a certain degree, its free radical clearance rate was similar to that of vitamin C control group. The order of different chemical contributions of constituents to the antioxidant activity in the fingerprint was 4>3>33>53>9>10>11>34>15>59>8>61>52>20>42>18>29. The preliminary exploration for the spectrum efficiency relations provides reference for studying traditional Chinese medicine compound processing method and the pharmacodyamic material basis.


DPPH ; antioxidant ; fingerprint ; grey relational analysis ; spectrum efficiency relations

[Indexed for MEDLINE]

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