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Sensors (Basel). 2019 May 31;19(11). pii: E2511. doi: 10.3390/s19112511.

Colorimetric Analysis of Glucose Oxidase-Magnetic Cellulose Nanocrystals (CNCs) for Glucose Detection.

Author information

1
School of Biological Sciences, Universiti Sains Malaysia, Penang 11700, Malaysia. yychuin@gmail.com.
2
Division of Bioresource, Paper and Coatings Technology, School of Industrial Technology, Universiti Sains Malaysia, Penang 11700, Malaysia. hrokiah@usm.my.
3
School of Biological Sciences, Universiti Sains Malaysia, Penang 11700, Malaysia. armyahya@usm.my.
4
School of Biological Sciences, Universiti Sains Malaysia, Penang 11700, Malaysia. ybustami@usm.my.

Abstract

Glucose oxidase (EC 1.1.3.4) sensors that have been developed and widely used for glucose monitoring have generally relied on electrochemical principle. In this study, the potential use of colorimetric method for glucose detection utilizing glucose oxidase-magnetic cellulose nanocrystals (CNCs) is explored. Magnetic cellulose nanocrystals (magnetic CNCs) were fabricated using iron oxide nanoparticles (IONPs) and cellulose nanocrystals (CNCs) via electrostatic self-assembly technique. Glucose oxidase was successfully immobilized on magnetic CNCs using carbodiimide-coupling reaction. About 33% of GOx was successfully attached on magnetic CNCs, and the affinity of GOx-magnetic CNCs to glucose molecules was slightly higher than free enzymes. Furthermore, immobilization does not affect the specificity of GOx-magnetic CNCs towards glucose and can detect glucose from 0.25 mM to 2.5 mM. Apart from that, GOx-magnetic CNCs stored at 4 °C for 4 weeks retained 70% of its initial activity and can be recycled for at least ten consecutive cycles.

KEYWORDS:

cellulose nanocrystals; detection; glucose oxidase; immobilization; iron oxide nanoparticles; magnetic cellulose nanocrystals

PMID:
31159318
PMCID:
PMC6603789
DOI:
10.3390/s19112511
[Indexed for MEDLINE]
Free PMC Article

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