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Sensors (Basel). 2018 May 10;18(5). pii: E1507. doi: 10.3390/s18051507.

High-Speed Lateral Flow Strategy for a Fast Biosensing with an Improved Selectivity and Binding Affinity.

Author information

1
Department of Physics and Astronomy and Institute of Applied Physics, Seoul National University, Seoul 08826, Korea. eastmania@snu.ac.kr.
2
Department of Physics and Astronomy and Institute of Applied Physics, Seoul National University, Seoul 08826, Korea. h30461@gmail.com.
3
Department of Chemical & Biological Engineering, KU-KIST Graduate School of Converging Science & Technology, Korea University, Seoul 02841, Korea. godls5933@naver.com.
4
Department of Chemical & Biological Engineering, KU-KIST Graduate School of Converging Science & Technology, Korea University, Seoul 02841, Korea. kyo_choi@korea.ac.kr.
5
Department of Physics and Astronomy and Institute of Applied Physics, Seoul National University, Seoul 08826, Korea. m201320376@snu.ac.kr.
6
Department of Chemical & Biological Engineering, KU-KIST Graduate School of Converging Science & Technology, Korea University, Seoul 02841, Korea. ahn@korea.ac.kr.
7
Department of Physics and Astronomy and Institute of Applied Physics, Seoul National University, Seoul 08826, Korea. seunghun@snu.ac.kr.

Abstract

We report a high-speed lateral flow strategy for a fast biosensing with an improved selectivity and binding affinity even under harsh conditions. In this strategy, biosensors were fixed at a location away from the center of a round shape disk, and the disk was rotated to create the lateral flow of a target solution on the biosensors during the sensing measurements. Experimental results using the strategy showed high reaction speeds, high binding affinity, and low nonspecific adsorptions of target molecules to biosensors. Furthermore, binding affinity between target molecules and sensing molecules was enhanced even in harsh conditions such as low pH and low ionic strength conditions. These results show that the strategy can improve the performance of conventional biosensors by generating high-speed lateral flows on a biosensor surface. Therefore, our strategy can be utilized as a simple but powerful tool for versatile bio and medical applications.

KEYWORDS:

binding affinity; lateral flow; reaction speed; rotating disk; selectivity

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