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Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Jun;29(6):1668-71.

[Analysis of the extraction of red pellicle of walnut (Juglans regia L.) by ultraviolet-visible spectra and HPLC-ESI-MS(n)].

[Article in Chinese]

Author information

1
College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China. wangkejian7212@263.net

Abstract

The extraction of the red pellicle of walnut (Juglans regia L.) was analyzed by UV-visible spectra and HPLC-ESI-MS(n) (high-performance-liquid-chromatography coupled with electrospray ionization mass spectrometry). The extraction in ethanol-HCl showed two absorption peaks at 560 and 591 nm respectively in the UV-Vis spectrum; after purified by lead acetate and thin-layer-chromatography, the extraction in ethanol-HCl showed 4 absorption peaks at 340, 370, 552 and 585 nm respectively. These results testified that the anthocyanin was in the extraction. Six molecular ion peaks (m/z) occurred on MS: 301, 481, 633, 783, 785 and 950, which was identified as ellagic acid, Hexahydroxydiphenoyl(HHDP)-glucose, Galloyl-HHDP-glucose, Di-HHDP-glucose, Di-Galloyl-HHDP-glucose, and HHDP-Valoneoyl-glucose respectively.

PMID:
19810556
[Indexed for MEDLINE]

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