Format

Send to

Choose Destination
Molecules. 2015 Jul 2;20(7):12114-24. doi: 10.3390/molecules200712114.

Quantitative 1H-NMR Method for the Determination of Tadalafil in Bulk Drugs and its Tablets.

Author information

1
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical Colledge, Beijing 100050, China. yqy@imm.ac.cn.
2
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical Colledge, Beijing 100050, China. qiuhui@imm.ac.cn.
3
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical Colledge, Beijing 100050, China. guowei@imm.ac.cn.
4
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical Colledge, Beijing 100050, China. wangdmchina@imm.ac.cn.
5
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical Colledge, Beijing 100050, China. xiaodou_zxn@aliyun.com.
6
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical Colledge, Beijing 100050, China. xuedan0628@126.com.
7
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical Colledge, Beijing 100050, China. zhjl@imm.ac.cn.
8
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical Colledge, Beijing 100050, China. ws@imm.ac.cn.
9
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical Colledge, Beijing 100050, China. wyh@imm.ac.cn.

Abstract

A simple, rapid, accurate, and selective quantitative nuclear magnetic resonance method for the determination of tadalafil in bulk drugs and its tablets was established and evaluated. Spectra were obtained in dimethylsulfoxide-d6 using 2,4-dinitrotoluene as the internal standard. In this study, the method's linearity, range, limit of quantification, stability, precision, and accuracy were validated. The results were consistent with those obtained from high-performance liquid chromatography analysis. Thus, the proposed method is a useful and practical tool for the determination of tadalafil in bulk drugs and its tablets.

KEYWORDS:

2,4-dinitrotoluene; quantitative analysis; quantitative proton nuclear magnetic resonance; tadalafil

PMID:
26147583
PMCID:
PMC6332277
DOI:
10.3390/molecules200712114
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center