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Nutrients. 2017 Jul 6;9(7). pii: E706. doi: 10.3390/nu9070706.

Secretory Leukoprotease Inhibitor (Slpi) Expression Is Required for Educating Murine Dendritic Cells Inflammatory Response Following Quercetin Exposure.

Author information

1
National Institute of Gastroenterology "S. de Bellis", Institute of Research, Via Turi, 27, 70013 Castellana Grotte, Italy. stefania.desantis@ispa.cnr.it.
2
Institute of Sciences of Food Production C.N.R., Unit of Lecce, via Monteroni, 73100 Lecce, Italy. stefania.desantis@ispa.cnr.it.
3
National Institute of Gastroenterology "S. de Bellis", Institute of Research, Via Turi, 27, 70013 Castellana Grotte, Italy. vanessa.galleggiante@libero.it.
4
National Institute of Gastroenterology "S. de Bellis", Institute of Research, Via Turi, 27, 70013 Castellana Grotte, Italy. lety.scandiffio@hotmail.it.
5
National Institute of Gastroenterology "S. de Bellis", Institute of Research, Via Turi, 27, 70013 Castellana Grotte, Italy. marinaliso@libero.it.
6
Department of Pharmacy, School of Pharmacy, University of Salerno, 84084 Fisciano, Italy. esommella@unisa.it.
7
University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK. a.sobolewski@uea.ac.uk.
8
National Institute of Gastroenterology "S. de Bellis", Institute of Research, Via Turi, 27, 70013 Castellana Grotte, Italy. vito.spilotro@irccsdebellis.it.
9
Department of Pharmacy, School of Pharmacy, University of Salerno, 84084 Fisciano, Italy. pintoal@unisa.it.
10
Department of Pharmacy, School of Pharmacy, University of Salerno, 84084 Fisciano, Italy. pcampiglia@unisa.it.
11
European Biomedical Research Institute of Salerno (EBRIS), Via S. de Renzi, 3, 84125 Salerno, Italy. pcampiglia@unisa.it.
12
National Institute of Gastroenterology "S. de Bellis", Institute of Research, Via Turi, 27, 70013 Castellana Grotte, Italy. grazia.serino@irccsdebellis.it.
13
Institute of Sciences of Food Production C.N.R., Unit of Lecce, via Monteroni, 73100 Lecce, Italy. angelo.santino@ispa.cnr.it.
14
National Institute of Gastroenterology "S. de Bellis", Institute of Research, Via Turi, 27, 70013 Castellana Grotte, Italy. maria.notarnicola@irccsdebellis.it.
15
National Institute of Gastroenterology "S. de Bellis", Institute of Research, Via Turi, 27, 70013 Castellana Grotte, Italy. transmed@irccsdebellis.it.
16
Department of Pharmacy, School of Pharmacy, University of Salerno, 84084 Fisciano, Italy. transmed@irccsdebellis.it.

Abstract

Dendritic cells' (DCs) ability to present antigens and initiate the adaptive immune response confers them a pivotal role in immunological defense against hostile infection and, at the same time, immunological tolerance towards harmless components of the microbiota. Food products can modulate the inflammatory status of intestinal DCs. Among nutritionally-derived products, we investigated the ability of quercetin to suppress inflammatory cytokines secretion, antigen presentation, and DCs migration towards the draining lymph nodes. We recently identified the Slpi expression as a crucial checkpoint required for the quercetin-induced inflammatory suppression. Here we demonstrate that Slpi-KO DCs secrete a unique panel of cytokines and chemokines following quercetin exposure. In vivo, quercetin-enriched food is able to induce Slpi expression in the ileum, while little effects are detectable in the duodenum. Furthermore, Slpi expressing cells are more frequent at the tip compared to the base of the intestinal villi, suggesting that quercetin exposure could be more efficient for DCs projecting periscopes in the intestinal lumen. These data suggest that quercetin-enriched nutritional regimes may be efficient for suppressing inflammatory syndromes affecting the ileo-colonic tract.

KEYWORDS:

Slpi; dendritic cells; inflammation; quercetin

PMID:
28684695
PMCID:
PMC5537821
DOI:
10.3390/nu9070706
[Indexed for MEDLINE]
Free PMC Article

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