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Chem Biol Interact. 2015 Feb 25;228:79-87. doi: 10.1016/j.cbi.2015.01.023. Epub 2015 Jan 22.

Plasma lipidomics reveal profound perturbation of glycerophospholipids, fatty acids, and sphingolipids in diet-induced hyperlipidemia.

Author information

1
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, No. 229 Taibai North Road, Xi'an, Shaanxi 710069, China; Division of Nephrology and Hypertension, School of Medicine, University of California, Irvine, Med Sci I, C352, UCI Campus, Irvine, CA 92897, USA.
2
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, No. 229 Taibai North Road, Xi'an, Shaanxi 710069, China.
3
Department of Computer Science and Engineering, University of Shanghai for Science and Technology, No. 516 Jungong Road, Shanghai 200093, China.
4
Solution Centre, Waters Technologies (Shanghai) Ltd., No. 1000 Jinhai Road, Shanghai 201203, China.
5
Division of Nephrology and Hypertension, School of Medicine, University of California, Irvine, Med Sci I, C352, UCI Campus, Irvine, CA 92897, USA. Electronic address: ndvaziri@uci.edu.
6
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, No. 229 Taibai North Road, Xi'an, Shaanxi 710069, China; Division of Nephrology and Hypertension, School of Medicine, University of California, Irvine, Med Sci I, C352, UCI Campus, Irvine, CA 92897, USA. Electronic address: zyy@nwu.edu.cn.

Abstract

Hyperlipidemia is a major risk factor for coronary heart disease and has emerged as an important public health problem. Lipidomics is a powerful technology for assessment of global lipid metabolites in a biological system and for biomarker discovery. In the present study, hyperlipidemia was induced by feeding rats a high fat diet. A sensitive ultra-performance liquid chromatography coupled with quadrupole time-of-flight synapt high-definition mass spectrometry method was used for the analysis of plasma lipids. Orthogonal partial least squares-discriminant analysis, correlation analysis and heatmap analysis were performed to investigate the metabolic changes in rats with diet-induced hyperlipidemia. Potential biomarkers were detected using S-plot and were identified by accurate mass data, isotopic pattern and MS(E) fragments information. Significantly increased total cholesterol, triglycerides and low-density lipoprotein cholesterol as well as decreased high-density lipoprotein cholesterol were observed in diet-induced hyperlipidemic rats. Combined with standard serum biochemical results, significant differences in plasma lipid compounds including eleven glycerophospholipids, six fatty acids, two sphingolipids, one eicosanoid, one sterol lipid and one glycerolipid were observed, highlighting the perturbation of lipid metabolism in diet-induced hyperlipidemia. These findings provide further insights into the lipid profile across a wide range of biochemical pathways in diet-induced hyperlipidemia.

KEYWORDS:

High fat diet; High-definition mass spectrometry; Hyperlipidemia; Lipid abnormality; Lipidomics; Ultra-performance liquid chromatography

PMID:
25619641
DOI:
10.1016/j.cbi.2015.01.023
[Indexed for MEDLINE]

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