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Toxins (Basel). 2016 Nov 29;8(12). pii: E355.

Fast Screening of Antibacterial Compounds from Fusaria.

Author information

1
Department of Chemistry and Bioscience, Aalborg University, Frederik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. tes@bio.aau.dk.
2
Department of Animal Science, Faculty of Science and Technology, Aarhus University, Blichers Allé 20, DK-8830 Tjele, Denmark. marlene.fredborg@anis.au.dk.
3
Department of Chemistry and Bioscience, Aalborg University, Frederik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. a-mariac@hotmail.com.
4
Department of Chemistry and Bioscience, Aalborg University, Frederik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. baldurskd@hotmail.com.
5
Department of Chemistry and Bioscience, Aalborg University, Frederik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. nikolinekramer@hotmail.com.
6
Department of Chemistry and Bioscience, Aalborg University, Frederik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. hgiese@bio.aau.dk.
7
Department of Chemistry and Bioscience, Aalborg University, Frederik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. jls@bio.aau.dk.
8
Department of Chemistry and Bioscience, Aalborg University, Niels Bohrs Vej 8, 6700 Esbjerg, Denmark. jls@bio.aau.dk.

Abstract

Bio-guided screening is an important method to identify bioactive compounds from fungi. In this study we applied a fast digital time-lapse microscopic method for assessment of the antibacterial properties of secondary metabolites from the fungal genus Fusarium. Here antibacterial effects could be detected for antibiotic Y, aurofusarin, beauvericin, enniatins and fusaric acid after six hours of cultivation. The system was then used in a bio-guided screen of extracts from 14 different Fusarium species, which had been fractionated by HPLC. In this screen, fractions containing the red pigments aurofusarin and bikaverin showed effects against strains of Lactobacillus and Bifidobacterium. The IC50 for aurofusarin against Lactobacillus acidophilus was 8 µM, and against Bifidobacterium breve it was 64 µM. Aurofusarin only showed an effect on probiotic bacteria, leading to the speculation that only health-promoting bacteria with a positive effect in the gut system are affected.

KEYWORDS:

Fusarium; antibacterial; antibiotics; aurofusarin; bio-guided assays; bioactivity; mycotoxins; pigments; polyketides; secondary metabolites

PMID:
27916854
PMCID:
PMC5198549
DOI:
10.3390/toxins8120355
[Indexed for MEDLINE]
Free PMC Article

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