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Biosensors (Basel). 2019 Jan 13;9(1). pii: E12. doi: 10.3390/bios9010012.

Biochemical Changes in Irradiated Oral Mucosa: A FTIR Spectroscopic Study.

Author information

1
Research Group of Oral Health Sciences, Faculty of Medicine, University of Oulu, and Oulu University Hospital, P.O. Box 8000, 90014 Oulu, Finland. Helena.Ukkonen@oulu.fi.
2
Medical Research Center Oulu, Oulu University Hospital, P.O. Box 5000, 90029 Oulu, Finland. Helena.Ukkonen@oulu.fi.
3
SIB Labs, University of Eastern Finland, P.O. Box 1627, 70211 Kuopio, Finland. Helena.Ukkonen@oulu.fi.
4
SIB Labs, University of Eastern Finland, P.O. Box 1627, 70211 Kuopio, Finland. simovuo@student.uef.fi.
5
Institute of Dentistry, University of Eastern Finland, Kuopio campus, and Educational Dental Clinic, Kuopio University Hospital, P.O. Box 1627, 70211 Kuopio, Finland. simovuo@student.uef.fi.
6
SIB Labs, University of Eastern Finland, P.O. Box 1627, 70211 Kuopio, Finland. jopi.mikkonen@uef.fi.
7
Institute of Dentistry, University of Eastern Finland, Kuopio campus, and Educational Dental Clinic, Kuopio University Hospital, P.O. Box 1627, 70211 Kuopio, Finland. jopi.mikkonen@uef.fi.
8
Amsterdam UMC and Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit Amsterdam, Department of Oral and Maxillofacial Surgery/Oral Pathology, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands. ha.dekker@vumc.nl.
9
Amsterdam UMC and Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit Amsterdam, Department of Oral and Maxillofacial Surgery/Oral Pathology, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands. eajm.schulten@vumc.nl.
10
Amsterdam UMC and Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit Amsterdam, Department of Oral and Maxillofacial Surgery/Oral Pathology, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands. e.bloemena@vumc.nl.
11
SIB Labs, University of Eastern Finland, P.O. Box 1627, 70211 Kuopio, Finland. arto.koistinen@uef.fi.
12
Department of Otolaryngology, School of Medicine, Stanford University, Patlo Alto, CA 94305, USA. tvaldez1@stanford.edu.
13
Research Group of Oral Health Sciences, Faculty of Medicine, University of Oulu, and Oulu University Hospital, P.O. Box 8000, 90014 Oulu, Finland. arja.kullaa@uef.fi.
14
Institute of Dentistry, University of Eastern Finland, Kuopio campus, and Educational Dental Clinic, Kuopio University Hospital, P.O. Box 1627, 70211 Kuopio, Finland. arja.kullaa@uef.fi.
15
SIB Labs, University of Eastern Finland, P.O. Box 1627, 70211 Kuopio, Finland. spsingh1@stanford.edu.
16
Institute of Dentistry, University of Eastern Finland, Kuopio campus, and Educational Dental Clinic, Kuopio University Hospital, P.O. Box 1627, 70211 Kuopio, Finland. spsingh1@stanford.edu.
17
Department of Otolaryngology, School of Medicine, Stanford University, Patlo Alto, CA 94305, USA. spsingh1@stanford.edu.

Abstract

Radiation exposure during the course of treatment in head and neck cancer (HNC) patients can induce both structural and biochemical anomalies. The present study is focused on utilizing infrared imaging for the identification of the minor biochemical alterations in the oral mucosa. Chemical maps generated using glycoprotein band indicates its differential distribution along the superficial layer. Spectra extracted from this layer suggests changes in overall nucleic acid and protein content in response to the therapeutic irradiation. Discrimination among control and irradiated groups have been achieved using principal component analysis. Findings of this preliminary study further support prospective utilization of Fourier Transform InfraRed (FTIR) imaging as a non-destructive, label-free tool for objective assessment of the oral mucosa in patient groups with or without radiation therapy.

KEYWORDS:

FTIR spectroscopy; head and neck cancer; radiotherapy

PMID:
30642117
PMCID:
PMC6468735
DOI:
10.3390/bios9010012
[Indexed for MEDLINE]
Free PMC Article

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