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Int J Mol Sci. 2019 Oct 29;20(21). pii: E5394. doi: 10.3390/ijms20215394.

Antibacterial Property of Composites of Reduced Graphene Oxide with Nano-Silver and Zinc Oxide Nanoparticles Synthesized Using a Microwave-Assisted Approach.

Author information

1
Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 81143, Taiwan. yihhsueh@nkust.edu.tw.
2
Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan 32003, Taiwan. cthsieh@saturn.yzu.edu.
3
Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan 32003, Taiwan. qcricnt@hotmail.com.
4
Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Taoyuan 32003, Taiwan. n8808701@gmail.com.
5
Department of Internal Medicine, Far Eastern Memorial Hospital, New Taipei City 220, Taiwan. chiaying.liu@gmail.com.
6
Graduate Institute of Biomedical Sciences, and Research Center for Bacterial Pathogenesis, Chang Gung University, Taoyuan 33302, Taiwan. d8901005@stmail.cgu.edu.tw.

Abstract

Graphene oxide (GO) composites with various metal nanoparticles (NPs) are attracting increasing interest owing to their broad scope in biomedical applications. Here, microwave-assisted chemical reduction was used to deposit nano-silver and zinc oxide NPs (Ag and ZnO NPs) on the surface of reduced GO (rGO) at the following weight percentages: 5.34% Ag/rGO, 7.49% Ag/rGO, 6.85% ZnO/rGO, 16.45% ZnO/rGO, 3.47/34.91% Ag/ZnO/rGO, and 7.08/15.28% Ag/ZnO/rGO. These materials were tested for antibacterial activity, and 3.47/34.91% Ag/ZnO/rGO and 7.08/15.28% Ag/ZnO/rGO exhibited better antibacterial activity than the other tested materials against the gram-negative bacterium Escherichia coli K12. At 1000 ppm, both these Ag/ZnO/rGO composites had better killing properties against both E. coli K12 and the gram-positive bacterium Staphylococcus aureus SA113 than Ag/rGO and ZnO/rGO did. RedoxSensor flow cytometry showed that 3.47/34.91% Ag/ZnO/rGO and 7.08/15.28% Ag/ZnO/rGO decreased reductase activity and affected membrane integrity in the bacteria. At 100 ppm, these two composites affected membrane integrity more in E. coli, while 7.08/15.28% Ag/ZnO/rGO considerably decreased reductase activity in S. aureus. Thus, the 3.47/34.91% and 7.08%/15.28% Ag/ZnO/rGO nanocomposites can be applied not only as antibacterial agents but also in a variety of biomedical materials such as sensors, photothermal therapy, drug delivery, and catalysis, in the future.

KEYWORDS:

Escherichia coli; Staphylococcus aureus; antibacterial agents; graphene; minimum inhibitory concentration; nano-silver; nanocomposite; sterilization

PMID:
31671904
DOI:
10.3390/ijms20215394
Free PMC Article

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