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Molecules. 2018 Aug 17;23(8). pii: E2068. doi: 10.3390/molecules23082068.

Beneficial Effect of Herbal Formulation KM1608 on Inflammatory Bowl Diseases: A Preliminary Experimental Study.

Author information

1
College of Korean Medicine, Gachon University, Seongnam-si, Gyeonggi-do 13120, Korea. ms.shin@gachon.ac.kr.
2
New Drug Research Team, Kolmar, Korea Co. Ltd, Sandan-gil, Jeonui-myeon, Sejong-si 30003, Korea. m302@kolmar.co.kr.
3
College of Korean Medicine, Gachon University, Seongnam-si, Gyeonggi-do 13120, Korea. jaemin.lee426@gmail.com.
4
New Drug Research Team, Kolmar, Korea Co. Ltd, Sandan-gil, Jeonui-myeon, Sejong-si 30003, Korea. fcosmos@kolmar.co.kr.
5
New Drug Research Team, Kolmar, Korea Co. Ltd, Sandan-gil, Jeonui-myeon, Sejong-si 30003, Korea. jimpark@kolmar.co.kr.
6
Department of Food Science and Biotechnology, Kyonggi University, Suwon-si, Gyeonggi-do 16227, Korea. rhemf@hanmail.net.
7
Department of Life Science, College of Bio-Nano Technology, Gachon University, Seongnam-si, Gyeonggi-do 13120, Korea. sullimlee@gachon.ac.kr.
8
College of Korean Medicine, Gachon University, Seongnam-si, Gyeonggi-do 13120, Korea. seoul@gachon.ac.kr.
9
New Drug Research Team, Kolmar, Korea Co. Ltd, Sandan-gil, Jeonui-myeon, Sejong-si 30003, Korea. bakoo9@kolmar.co.kr.
10
College of Korean Medicine, Gachon University, Seongnam-si, Gyeonggi-do 13120, Korea. kkang@gachon.ac.kr.

Abstract

Aucklandia lappa DC., Terminalia chebula Retz and Zingiber officinale Roscoe have been traditionally used in east Asia to treat chronic diarrhea and abdominal pain. This study aimed to evaluated the anti-inflammatory activity of KM1608, which is composed of three natural herbs in a mouse model of dextran sodium sulfate (DSS)-induced ulcerative colitis. The anti-inflammatory activity and underlying mechanism were assessed in vitro using LPS-treated RAW264.7 cells. The in vivo effect of KM1608 on DSS-induced colitis was examined after oral administration in mice. KM1608 significantly inhibited the inflammatory mediators such as nitric oxide, interleukin (IL)-6, monocyte chemotactic protein 1 (MCP-1) and tumor necrosis factor (TNF)-α in LPS-treated RAW264.7 cells. The inhibitory effect of KM1608 was attributed to the reduction of Akt phosphorylation in the LPS-treated cells. In the mouse model, oral administration of KM1608 significantly improved DSS-induced colitis symptoms, such as disease activity index (DAI), colon length, and colon weight, as well as suppressed the expression of IL-6, TNF-α, and myeloperoxidase (MPO) in the DSS-induced colitis tissues. Taken together, KM1608 improved colitis through the regulation of inflammatory responses, suggesting that KM1608 has potential therapeutic use in the treatment of inflammatory diseases.

KEYWORDS:

Aucklandia lappa DC.; Terminalia chebula Retz.; Zingiber officinale Roscoe; anti-inflammatory; ulcerative colitis

PMID:
30126158
DOI:
10.3390/molecules23082068
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