Format

Send to

Choose Destination
Viruses. 2019 Jul 13;11(7). pii: E644. doi: 10.3390/v11070644.

A Lineage of Begomoviruses Encode Rep and AC4 Proteins of Enigmatic Ancestry: Hints on the Evolution of Geminiviruses in the New World.

Author information

1
División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, A.C., Camino a la Presa de San José 2055, Lomas 4ta Secc, San Luis Potosi 78216, S.L.P., México.
2
Facultad de Ciencias Forestales, Universidad Juárez del Estado de Durango, Río Papaloapan Esquina con Blvd Durango (s/n), Col. Valle del Sur. 34120, Durango, Dgo, México.
3
Centro de Investigación Científica de Yucatán, A.C., Mérida 97000, Yucatán, México.
4
CONACYT-CIIDZA-Instituto Potosino de Investigación Científica y Tecnológica A.C., Camino a la Presa de San José 2055, Lomas 4ta Secc, San Luis Potosi 78216, S.L.P., México.
5
CONACyT-Universidad de Colima, Laboratorio de Agrobiotecnología, Carretera Los Limones-Loma de Juarez (s/n), Tecnoparque CLQ Colima 28629, Colima, México.
6
División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, A.C., Camino a la Presa de San José 2055, Lomas 4ta Secc, San Luis Potosi 78216, S.L.P., México. grarguel@ipicyt.edu.mx.

Abstract

The begomoviruses (BGVs) are plant pathogens that evolved in the Old World during the Cretaceous and arrived to the New World (NW) in the Cenozoic era. A subgroup of NW BGVs, the "Squash leaf curl virus (SLCV) lineage" (S-Lin), includes viruses with unique characteristics. To get clues on the evolutionary origin of this lineage, a search for divergent members was undertaken. Four novel BGVs were characterized, including one that is basal to the group. Comparative analyses led to discover a ~670 bp genome module that is nearly exclusive of this lineage, encompassing the replication origin, the AC4 gene, and 480 bp of the Rep gene. A similar DNA module was found in two curtoviruses, hence suggesting that the S-Lin ancestor acquired its distinctive genomic segment by recombination with a curtovirus. This hypothesis was definitely disproved by an in-depth sequence analysis. The search for homologs of S-Lin Rep uncover the common origin of Rep proteins encoded by diverse Geminiviridae genera and viral "fossils" integrated at plant genomes. In contrast, no homolog of S-Lin Rep was found in public databases. Consequently, it was concluded that the SLCV clade ancestor evolved by a recombination event between a primitive NW BGV and a virus from a hitherto unknown lineage.

KEYWORDS:

curtovirus taxonomy; endogenous viral element; geminivirus evolution; replication origin; replication protein; rolling-circle replication

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center