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Items: 1 to 20 of 135

1.

Cooperativity of Covalent Attachment and Ion Exchange on Alcalase Immobilization Using Glutaraldehyde Chemistry: Enzyme Stabilization and Improved Proteolytic Activity.

Ait Braham S, Hussain F, Morellon-Sterling R, Kamal S, Kornecki JF, Barbosa O, Kati DE, Fernandez-Lafuente R.

Biotechnol Prog. 2018 Dec 21. doi: 10.1002/btpr.2768. [Epub ahead of print]

PMID:
30575340
2.

Further Stabilization of Alcalase Immobilized on Glyoxyl Supports: Amination Plus Modification with Glutaraldehyde.

Hussain F, Arana-Peña S, Morellon-Sterling R, Barbosa O, Braham SA, Kamal S, Fernandez-Lafuente R.

Molecules. 2018 Dec 3;23(12). pii: E3188. doi: 10.3390/molecules23123188.

3.

Evaluation of Strategies to Produce Highly Porous Cross-Linked Aggregates of Porcine Pancreas Lipase with Magnetic Properties.

Guimarães JR, Giordano RLC, Fernandez-Lafuente R, Tardioli PW.

Molecules. 2018 Nov 16;23(11). pii: E2993. doi: 10.3390/molecules23112993.

4.
5.

Bioprocess development for biolubricant production using microbial oil derived via fermentation from confectionery industry wastes.

Papadaki A, Fernandes KV, Chatzifragkou A, Aguieiras ECG, da Silva JAC, Fernandez-Lafuente R, Papanikolaou S, Koutinas A, Freire DMG.

Bioresour Technol. 2018 Nov;267:311-318. doi: 10.1016/j.biortech.2018.07.016. Epub 2018 Jul 6.

PMID:
30029176
6.

Design of Bactericidal Peptides Against Escherichia coli O157:H7, Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus.

Cruz J, Rondon-Villarreal P, Torres RG, Urquiza M, Guzman F, Alvarez C, Abengozar MA, Sierra DA, Rivas L, Fernandez-Lafuente R, Ortiz CC.

Med Chem. 2018;14(7):741-752. doi: 10.2174/1573406414666180508120024.

PMID:
29737262
7.

Enzymatic synthesis of ethyl esters from waste oil using mixtures of lipases in a plug-flow packed-bed continuous reactor.

Poppe JK, Matte CR, de Freitas VO, Fernandez-Lafuente R, Rodrigues RC, Záchia Ayub MA.

Biotechnol Prog. 2018 Jul;34(4):952-959. doi: 10.1002/btpr.2650. Epub 2018 Jul 27.

PMID:
29708648
8.

1,3-Regiospecific ethanolysis of soybean oil catalyzed by crosslinked porcine pancreas lipase aggregates.

Ramos MD, Miranda LP, Giordano RLC, Fernandez-Lafuente R, Kopp W, Tardioli PW.

Biotechnol Prog. 2018 Jul;34(4):910-920. doi: 10.1002/btpr.2636. Epub 2018 May 7.

PMID:
29693317
9.

Transesterification of Waste Frying Oil and Soybean Oil by Combi-lipases Under Ultrasound-Assisted Reactions.

Poppe JK, Matte CR, Fernandez-Lafuente R, Rodrigues RC, Ayub MAZ.

Appl Biochem Biotechnol. 2018 Nov;186(3):576-589. doi: 10.1007/s12010-018-2763-x. Epub 2018 Apr 21.

PMID:
29680990
10.

Performance of Different Immobilized Lipases in the Syntheses of Short- and Long-Chain Carboxylic Acid Esters by Esterification Reactions in Organic Media.

de Lima LN, Mendes AA, Fernandez-Lafuente R, Tardioli PW, Giordano RLC.

Molecules. 2018 Mar 27;23(4). pii: E766. doi: 10.3390/molecules23040766.

11.

Kinetic resolution of drug intermediates catalyzed by lipase B from Candida antarctica immobilized on immobead-350.

Pinheiro MP, Rios NS, Fonseca TS, Bezerra FA, Rodríguez-Castellón E, Fernandez-Lafuente R, Carlos de Mattos M, Dos Santos JCS, Gonçalves LRB.

Biotechnol Prog. 2018 Jul;34(4):878-889. doi: 10.1002/btpr.2630. Epub 2018 Mar 30.

PMID:
29536666
12.

Stabilization of dimeric β-glucosidase from Aspergillus niger via glutaraldehyde immobilization under different conditions.

Vazquez-Ortega PG, Alcaraz-Fructuoso MT, Rojas-Contreras JA, López-Miranda J, Fernandez-Lafuente R.

Enzyme Microb Technol. 2018 Mar;110:38-45. doi: 10.1016/j.enzmictec.2017.12.007. Epub 2017 Dec 22.

PMID:
29310854
13.

Selective synthesis of partial glycerides of conjugated linoleic acids via modulation of the catalytic properties of lipases by immobilization on different supports.

Verdasco-Martín CM, Garcia-Verdugo E, Porcar R, Fernandez-Lafuente R, Otero C.

Food Chem. 2018 Apr 15;245:39-46. doi: 10.1016/j.foodchem.2017.10.072. Epub 2017 Oct 12.

PMID:
29287386
14.

Synthesis of Benzyl Acetate Catalyzed by Lipase Immobilized in Nontoxic Chitosan-Polyphosphate Beads.

Melo ADQ, Silva FFM, Dos Santos JCS, Fernández-Lafuente R, Lemos TLG, Dias Filho FA.

Molecules. 2017 Dec 7;22(12). pii: E2165. doi: 10.3390/molecules22122165.

15.

Improved stability of immobilized lipases via modification with polyethylenimine and glutaraldehyde.

Zaak H, Fernandez-Lopez L, Otero C, Sassi M, Fernandez-Lafuente R.

Enzyme Microb Technol. 2017 Nov;106:67-74. doi: 10.1016/j.enzmictec.2017.07.001. Epub 2017 Jul 6.

PMID:
28859812
16.

Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide.

Dos Santos JCS, Bonazza HL, de Matos LJBL, Carneiro EA, Barbosa O, Fernandez-Lafuente R, Gonçalves LRB, de Sant' Ana HB, Santiago-Aguiar RS.

Biotechnol Rep (Amst). 2017 Feb 28;14:16-26. doi: 10.1016/j.btre.2017.02.003. eCollection 2017 Mar.

17.

Special Issue: Enzyme Immobilization 2016.

Fernandez-Lafuente R.

Molecules. 2017 Apr 8;22(4). pii: E601. doi: 10.3390/molecules22040601. No abstract available.

18.

Effect of protein load on stability of immobilized enzymes.

Fernandez-Lopez L, Pedrero SG, Lopez-Carrobles N, Gorines BC, Virgen-Ortíz JJ, Fernandez-Lafuente R.

Enzyme Microb Technol. 2017 Mar;98:18-25. doi: 10.1016/j.enzmictec.2016.12.002. Epub 2016 Dec 16.

PMID:
28110660
19.

Desorption of Lipases Immobilized on Octyl-Agarose Beads and Coated with Ionic Polymers after Thermal Inactivation. Stronger Adsorption of Polymers/Unfolded Protein Composites.

Virgen-Ortíz JJ, Pedrero SG, Fernandez-Lopez L, Lopez-Carrobles N, Gorines BC, Otero C, Fernandez-Lafuente R.

Molecules. 2017 Jan 5;22(1). pii: E91. doi: 10.3390/molecules22010091.

20.

Relevance of substrates and products on the desorption of lipases physically adsorbed on hydrophobic supports.

Virgen-Ortíz JJ, Tacias-Pascacio VG, Hirata DB, Torrestiana-Sanchez B, Rosales-Quintero A, Fernandez-Lafuente R.

Enzyme Microb Technol. 2017 Jan;96:30-35. doi: 10.1016/j.enzmictec.2016.09.010. Epub 2016 Sep 20.

PMID:
27871382

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