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Molecules. 2019 Jul 10;24(14). pii: E2519. doi: 10.3390/molecules24142519.

Rapid Characterization of Triterpene Saponins from Zornia brasiliensis by HPLC-ESI-MS/MS.

Author information

1
Graduate Program in Natural and Synthetic Bioactive Products, Health Sciences Center, Universidade Federal da Paraíba, João Pessoa 58051-900, Paraíba, Brazil.
2
Graduate Program in Ecology and Conservation, Department of Biology, Center for Biological and Health Sciences, Campina Grande 58429-500, Paraíba, Brazil.
3
Center of Sciences and Technologies, Darcy Ribeiro Norte Fluminense State University, Campos dos Goytacazes 28013-600, Rio de Janeiro, Brazil.
4
Graduate Program in Natural and Synthetic Bioactive Products, Health Sciences Center, Universidade Federal da Paraíba, João Pessoa 58051-900, Paraíba, Brazil. josean@ltf.ufpb.br.

Abstract

Zornia brasiliensis Vogel (Leguminosae) is a species popularly known in Brazil as "urinária", "urinana", and "carrapicho", it is popularly used as a diuretic and in the treatment of venereal diseases. A specific methodology to obtain a saponin-enriched fraction and high-performance liquid chromatography coupled with diode array detection, ion trap mass spectrometry, and TOF-MS (HPLC-DAD-ESI-MS/MS) was applied for the analysis of triterpene saponins. The MS and MS/MS experiments were carried out by ionization in negative mode. Molecular mass and fragmentation data were used to support the structural characterization of the saponins. Based on retention times, high-resolution mass determination and fragmentation, 35 oleanane-triterpene saponins were tentatively identified in Z. brasiliensis.

KEYWORDS:

HPLC-DAD-ESI-MS/MS; Zornia brasiliensis; soyasaponins; triterpene saponins

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