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Genes (Basel). 2020 Feb 12;11(2). pii: E194. doi: 10.3390/genes11020194.

Identification, Expression, and Functions of the Somatostatin Gene Family in Spotted Scat (Scatophagus argus).

Feng P1,2, Tian C1,2,3, Lin X1,2, Jiang D1,2,3, Shi H1,2,3, Chen H1,2,3, Deng S1,2,3, Zhu C1,2,3, Li G1,2,3.

Author information

1
Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.
2
Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Zhanjiang 524088, China.
3
Marine Ecology and Aquaculture Environment of Zhanjiang, Zhanjiang 524088, China.

Abstract

Somatostatins (SSTs) are a family of proteins consisting of structurally diverse polypeptides that play important roles in the growth regulation in vertebrates. In the present study, four somatostatin genes (SST1, SST3, SST5, and SST6) were identified and characterized in the spotted scat (Scatophagus argus). The open reading frames (ORFs) of SST1, SST3, SST5, and SST6 cDNA consist of 372, 384, 321, and 333 bp, respectively, and encode proteins of 123, 127, 106, and 110 amino acids, respectively. Amino acid sequence alignments indicated that all SST genes contained conserved somatostatin signature motifs. Real-time PCR analysis showed that the SST genes were expressed in a tissue specific manner. When liver fragments were cultured in vitro with synthetic peptides (SST1, SST2, or SST6 at 1 μM or 10 μM) for 3 h or 6 h, the expression of insulin-like growth factor 1 and 2 (Igf-1 and Igf-2) in the liver decreased significantly. Treatment with SST5 had no significant effect on Igf-1 and Igf-2 gene expression. This study provides an enhanced understanding of the gene structure and expression patterns of the SST gene family in S. argus. Furthermore, this study provides a foundation for future exploration into the role of SST genes in growth and development.

KEYWORDS:

Scatophagus argus; somatostatin family; tissues expression; vitro incubation

Conflict of interest statement

The authors declare no conflicts of interest. The funders had no role in the design of the study, data collection, analyses, interpretation of data, in the preparation of the manuscript, or in the decision to publish the results.

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