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Environ Sci Pollut Res Int. 2019 Jul 22. doi: 10.1007/s11356-019-05958-6. [Epub ahead of print]

Genetic and systemic toxicity induced by silver and copper oxide nanoparticles, and their mixture in Clarias gariepinus (Burchell, 1822).

Author information

1
Cell Biology and Genetics Unit, Department of Zoology, University of Ibadan, Ibadan, Nigeria.
2
Drug Metabolism and Toxicology Research Laboratory, Department of Biochemistry, University of Ibadan, Ibadan, Nigeria.
3
Department of Biology, Federal University of Technology, Akure, Nigeria.
4
Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 41, rue du Brill, L-4422, Belvaux, Luxembourg.
5
Material Research and Technology (MRT) Department, Luxembourg Institute of Science and Technology (LIST), 41, rue du Brill, L-4422, Belvaux, Luxembourg.
6
Cell Biology and Genetics Unit, Department of Zoology, University of Ibadan, Ibadan, Nigeria. adebakar19@yahoo.com.

Abstract

Unanticipated increase in the use of silver (Ag) and copper oxide (CuO) nanoparticles (NPs) due to their antimicrobial properties is eliciting environmental health concern because of their coexistence in the aquatic environment. Therefore, we investigated the genetic and systemic toxicity of the individual NPs and their mixture (1:1) using the piscine micronucleus (MN) assay, haematological, histopathological (skin, gills and liver) and hepatic oxidative stress analyses [malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT)] in the African mud catfish, Clarias gariepinus. The fish were exposed to sublethal concentrations (6.25-100.00 mg/L) of each NP and their mixture for 28 days. Both NPs and their mixture induced significant (p < 0.05) increase in MN frequency and other nuclear abnormalities. There was significant decrease in haemoglobin concentration, red and white blood cell counts. Histopathological lesions observed include epidermal skin cells and gill lamellae hyperplasia and necrosis of hepatocytes. The levels of MDA, GSH and activities of SOD and CAT were impacted in C. gariepinus liver following the exposure to the NPs and their mixture. Interaction factor analysis of data indicates antagonistic genotoxicity and oxidative damage of the NPs mixture. These results suggest cytogenotoxic effects of Ag NPs, CuO NPs and their mixture via oxidative stress in Clarias gariepinus.

KEYWORDS:

Clarias gariepinus; Cytogenotoxicity; Haematology; Histopathology; Nanoparticles; Oxidative stress

PMID:
31332682
DOI:
10.1007/s11356-019-05958-6

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