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Biomedicines. 2019 Oct 21;7(4). pii: E83. doi: 10.3390/biomedicines7040083.

Increased Progesterone on the Day of Administration of hCG in Controlled Ovarian Hyperstimulation Affects the Expression of HOXA10 in Primates' Endometrial Receptivity.

Author information

1
Depatment of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia. nurhuda.ms@ui.ac.id.
2
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia. ninikbiokim@yahoo.com.
3
Depatment of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia. dwi.ari@ui.ac.id.
4
Biomedical Science Master Program, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia. andheadebbypradhita@gmail.com.
5
Biomedical Science Master Program, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia. ochathuffi@gmail.com.
6
Department of Pathology Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia. kusmardi.ms@ui.ac.id.

Abstract

The increase in progesterone (P4) levels on the day of human chorionic gonadotropin (hCG) administration have a negative effect on endometrial receptivity. There are few reports regarding the expression of homeobox A10 (HOXA10) as one of many biomolecular factors of endometrial receptivity. To evaluate the effect of increased P4 concentration on the day of hCG administration on HOXA10, a total of 16 Macaca nemestrina were divided into three dose groups of recombinant-follicle stimulating hormone (rFSH) (30IU, 50IU, and 70IU) and one control group. Injection of rFSH combined with gonadotropin release hormone (GnRH) at 160 ug/day was given subcutaneously using a long protocol technique. Blood samples for estradiol (E2) and (P4) concentration measurements were taken on the day of injecting hCG in the final follicular phase, while the collection of endometrial tissue for HOXA10 measurement was carried out 8 to 10 days after hCG administration. E2 and P4 were measured by ELISA, whereas HOXA10 expression was measured with immunohistochemical (IHC) techniques. The concentration of E2 and P4 was found to be higher in dose groups compared with the natural group, but no significant differences were found within the group. For the Hscore for HOXA10 expression, no significant differences within dose groups were found. In addition, no significant differences for the Hscore for HOXA10 were found when compared to E2 groups. Significantly, the Hscore of HOXA10 was found to be >1 ng/mL in the P4 group compared with the Hscore HOXA10 in the P4 natural group (p = 0.022). The high concentration of P4 caused by ovarian hyperstimulation in the follicular phase stimulates the expression of HOXA10 in the secretion phase.

KEYWORDS:

COH; HOXA10; endometrial receptivity; hCG administration; progesterone

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