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Biosensors (Basel). 2018 Jan 15;8(1). pii: E7. doi: 10.3390/bios8010007.

A Low-Cost Inkjet-Printed Aptamer-Based Electrochemical Biosensor for the Selective Detection of Lysozyme.

Author information

1
Department of Electrical and Computer Engineering, University of New Hampshire, Durham, NH 03824, USA. mk1125@wildcats.unh.edu.
2
Department of Chemical Engineering, University of New Hampshire, Durham, NH 03824, USA. agm11@wildcats.unh.edu.
3
Department of Electrical and Computer Engineering, University of New Hampshire, Durham, NH 03824, USA. edward.song@unh.edu.
4
Center for Advanced Materials and Manufacturing Innovation, University of New Hampshire, Durham, NH 03824, USA. edward.song@unh.edu.

Abstract

Recently, inkjet-printing has gained increased popularity in applications such as flexible electronics and disposable sensors, as well as in wearable sensors because of its multifarious advantages. This work presents a novel, low-cost immobilization technique using inkjet-printing for the development of an aptamer-based biosensor for the detection of lysozyme, an important biomarker in various disease diagnosis. The strong affinity between the carbon nanotube (CNT) and the single-stranded DNA is exploited to immobilize the aptamers onto the working electrode by printing the ink containing the dispersion of CNT-aptamer complex. The inkjet-printing method enables aptamer density control, as well as high resolution patternability. Our developed sensor shows a detection limit of 90 ng/mL with high target selectivity against other proteins. The sensor also demonstrates a shelf-life for a reasonable period. This technology has potential for applications in developing low-cost point-of-care diagnostic testing kits for home healthcare.

KEYWORDS:

aptamer; biosensor; electrochemical impedance spectroscopy; inkjet printing; lysozyme; point-of-care

PMID:
29342960
PMCID:
PMC5872055
DOI:
10.3390/bios8010007
[Indexed for MEDLINE]
Free PMC Article

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