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Toxics. 2017 Apr 11;5(2). pii: E11. doi: 10.3390/toxics5020011.

Vitis vinifera Extract Ameliorate Hepatic and Renal Dysfunction Induced by Dexamethasone in Albino Rats.

Author information

1
Biochemistry Department, College of Medicine, University of Hail, Hail 2440, Saudi Arabia. nabilhasona9@gmail.com.
2
Chemistry Department, Biochemistry Division, Faculty of Science, Beni-Suef University, Beni-Suef 62511, Egypt. nabilhasona9@gmail.com.
3
College of Medicine, University of Hail, Hail 2440, Saudi Arabia. celesa7med@gmail.com.
4
College of Medicine, University of Hail, Hail 2440, Saudi Arabia. thamer265@hotmail.com.
5
College of Medicine, University of Hail, Hail 2440, Saudi Arabia. aboshdkh@gmail.com.
6
Pharmacology Department, College of Medicine, University of Hail, Hail 2440, Saudi Arabia. clinical.cology@gmail.com.

Abstract

This study was conducted to evaluate the biochemical effects of grape seed extract against dexamethasone-induced hepatic and renal dysfunction in a female albino rat. Twenty-eight adult female rats were divided randomly into four equal groups: Group 1: animals were injected subcutaneously with saline and consider as normal control one. Group 2: animals were injected subcutaneously with dexamethasone in a dose of 0.1 mg/kg body weight. Group 3: animals were injected subcutaneously with 0.1 mg/kg body weight of dexamethasone, and then treated with a grape seed extract in a dose of 200 mg/kg body weight by oral gavage. Group 4: animals were injected subcutaneously with 0.1 mg/kg body weight of dexamethasone, and then treated with a grape seed extract in a dose of 400 mg/kg body weight by oral gavage. After 4 weeks, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) activities, albumin, uric acid, creatinine, and glucose levels were assayed. Hepatic reduced glutathione (GSH), total protein content, and catalase and glucose-6-phosphate dehydrogenase activities were also assayed. Dexamethasone administration caused elevation of serum levels of glucose, uric acid, creatinine, ALT, AST activities, and a decrease in other parameters such as hepatic glutathione, total protein levels, and catalase enzyme activity. Treatment with Vitis vinifera L. seed extract showed a significant increase in the body weight of rats in the group treated with Vitis vinifera L. seed extract orally compared with the dexamethasone control group. An increase in GSH and catalase activity in response to oral treatment with Vitis vinifera L. seed extract was observed after treatment. Grape seed extract positively affects glucocorticoid-induced hepatic and renal alteration in albino rats.

KEYWORDS:

GSH; alteration; catalase; dexamethasone; glucose-6-phosphate dehydrogenase; grape seed; hepatic

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