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Animals (Basel). 2020 Jan 29;10(2). pii: E219. doi: 10.3390/ani10020219.

Anthelmintic Activity of Wormwood (Artemisia absinthium L.) and Mallow (Malva sylvestris L.) against Haemonchus contortus in Sheep.

Author information

1
Institute of Animal Physiology, Centre of Biosciences of Slovak Academy of Sciences, 040 01 Košice, Slovakia.
2
Institute of Parasitology of Slovak Academy of Sciences, 040 01 Košice, Slovakia.
3
Medical University of Wroclaw, Department of Pharmaceutical Biology with Botanical Garden of Medicinal Plants, 50-556 Wroclaw, Poland.
4
Poznan University of Life Sciences, Department of Animal Nutrition, 60-637 Poznan, Poland.

Abstract

The objective of this study is to evaluate the effect of dry wormwood and mallow on the gastrointestinal parasite of small ruminants Haemonchus contortus. Twenty-four experimentally infected lambs were randomly divided into four groups of six animals each: unsupplemented lambs, lambs supplemented with wormwood, lambs supplemented with mallow and animals supplemented with a mix of both plants. Faecal samples from the lambs were collected on day 23, 29, 36, 43, 50, 57, 64 and 75 post-infection for quantification of the number of eggs per gram (EPG). The mix of both plants contained phenolic acids (10.7 g/kg DM) and flavonoids (5.51 g/kg DM). The nematode eggs were collected and in vitro egg hatch test was performed. The aqueous extracts of both plants exhibited strong ovicidal effect on H. contortus, with ED50 and ED99 values of 1.40 and 3.76 mg/mL and 2.17 and 5.89 mg/mL, respectively, in the in vitro tests. Despite the great individual differences between the treated lambs in eggs reduction, the mean EPG of the untreated and treated groups did not differ (p > 0.05). Our results indicate that using wormwood and mallow as dietary supplements do not have a sufficient effect on lambs infected with H. contortus.

KEYWORDS:

Haemonchus contortus; UHRMS; dietary treatments; egg counts; plant bioactive compounds

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