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Validation of the CULTEX® Radial Flow System for the assessment of the acute inhalation toxicity of airborne particles.

Tsoutsoulopoulos A, Gohlsch K, Möhle N, Breit A, Hoffmann S, Krischenowski O, Mückter H, Gudermann T, Thiermann H, Aufderheide M, Steinritz D.

Toxicol In Vitro. 2019 Aug;58:245-255. doi: 10.1016/j.tiv.2019.03.020. Epub 2019 Mar 16.


Is it the time to study air pollution effects under environmental conditions? A case study to support the shift of in vitro toxicology from the bench to the field.

Gualtieri M, Grollino MG, Consales C, Costabile F, Manigrasso M, Avino P, Aufderheide M, Cordelli E, Di Liberto L, Petralia E, Raschellà G, Stracquadanio M, Wiedensohler A, Pacchierotti F, Zanini G.

Chemosphere. 2018 Sep;207:552-564. doi: 10.1016/j.chemosphere.2018.05.130. Epub 2018 May 23.


In vitro exposure systems and dosimetry assessment tools for inhaled tobacco products: Workshop proceedings, conclusions and paths forward for in vitro model use.

Behrsing H, Hill E, Raabe H, Tice R, Fitzpatrick S, Devlin R, Pinkerton K, Oberdörster G, Wright C, Wieczorek R, Aufderheide M, Steiner S, Krebs T, Asgharian B, Corley R, Oldham M, Adamson J, Li X, Rahman I, Grego S, Chu PH, McCullough S, Curren R.

Altern Lab Anim. 2017 Jul;45(3):117-158.


Phenotypical changes in a differentiating immortalized bronchial epithelial cell line after exposure to mainstream cigarette smoke and e-cigarette vapor.

Aufderheide M, Emura M.

Exp Toxicol Pathol. 2017 Jul 5;69(6):393-401. doi: 10.1016/j.etp.2017.03.004. Epub 2017 Apr 1.


Improvement of the CULTEX® exposure technology by radial distribution of the test aerosol.

Aufderheide M, Heller WD, Krischenowski O, Möhle N, Hochrainer D.

Exp Toxicol Pathol. 2017 Jul 5;69(6):359-365. doi: 10.1016/j.etp.2017.02.004. Epub 2017 Mar 3.


Metaplastic phenotype in human primary bronchiolar epithelial cells after repeated exposure to native mainstream smoke at the air-liquid interface.

Aufderheide M, Ito S, Ishikawa S, Emura M.

Exp Toxicol Pathol. 2017 Jun 14;69(5):307-315. doi: 10.1016/j.etp.2017.01.015. Epub 2017 Feb 21.


Response to Polosa et al. Comments on Scheffler et al. Cytotoxic Evaluation of E-Liquid Aerosol Using Different Lung Derived Cell Models. Int. J. Environ. Res. Public Health, 2015, 12, 12466-12474.

Scheffler S, Dieken H, Krischenowski O, Aufderheide M.

Int J Environ Res Public Health. 2016 Jan 6;13(1). pii: E109. doi: 10.3390/ijerph13010109. No abstract available.


A new computer-controlled air-liquid interface cultivation system for the generation of differentiated cell cultures of the airway epithelium.

Aufderheide M, Förster C, Beschay M, Branscheid D, Emura M.

Exp Toxicol Pathol. 2016 Jan;68(1):77-87. doi: 10.1016/j.etp.2015.10.001. Epub 2015 Oct 24.


Cytotoxic Evaluation of e-Liquid Aerosol using Different Lung-Derived Cell Models.

Scheffler S, Dieken H, Krischenowski O, Aufderheide M.

Int J Environ Res Public Health. 2015 Oct 5;12(10):12466-74. doi: 10.3390/ijerph121012466.


Optimization of the CULTEX(®) radial flow system for in vitro investigation of lung damaging agents.

Tsoutsoulopoulos A, Möhle N, Aufderheide M, Schmidt A, Thiermann H, Steinritz D.

Toxicol Lett. 2016 Feb 26;244:28-34. doi: 10.1016/j.toxlet.2015.09.003. Epub 2015 Sep 7.


Ciliatoxicity in human primary bronchiolar epithelial cells after repeated exposure at the air-liquid interface with native mainstream smoke of K3R4F cigarettes with and without charcoal filter.

Aufderheide M, Scheffler S, Ito S, Ishikawa S, Emura M.

Exp Toxicol Pathol. 2015 Jul-Aug;67(7-8):407-11. doi: 10.1016/j.etp.2015.04.006. Epub 2015 May 8.


Evaluation of E-cigarette liquid vapor and mainstream cigarette smoke after direct exposure of primary human bronchial epithelial cells.

Scheffler S, Dieken H, Krischenowski O, Förster C, Branscheid D, Aufderheide M.

Int J Environ Res Public Health. 2015 Apr 8;12(4):3915-25. doi: 10.3390/ijerph120403915.


Target cell types with stem/progenitor function to isolate for in vitro reconstruction of human bronchiolar epithelia.

Emura M, Aufderheide M, Mohr U.

Exp Toxicol Pathol. 2015 Feb;67(2):81-8. doi: 10.1016/j.etp.2014.11.003. Epub 2014 Dec 4. Review.


Direct exposure at the air-liquid interface: evaluation of an in vitro approach for simulating inhalation of airborne substances.

Rach J, Budde J, Möhle N, Aufderheide M.

J Appl Toxicol. 2014 May;34(5):506-15. doi: 10.1002/jat.2899. Epub 2013 Jun 14.


Use of the Cultex® Radial Flow System as an in vitro exposure method to assess acute pulmonary toxicity of fine dusts and nanoparticles with special focus on the intra- and inter-laboratory reproducibility.

Steinritz D, Möhle N, Pohl C, Papritz M, Stenger B, Schmidt A, Kirkpatrick CJ, Thiermann H, Vogel R, Hoffmann S, Aufderheide M.

Chem Biol Interact. 2013 Dec 5;206(3):479-90. doi: 10.1016/j.cbi.2013.05.001. Epub 2013 May 11.


The CULTEX RFS: a comprehensive technical approach for the in vitro exposure of airway epithelial cells to the particulate matter at the air-liquid interface.

Aufderheide M, Halter B, Möhle N, Hochrainer D.

Biomed Res Int. 2013;2013:734137. doi: 10.1155/2013/734137. Epub 2013 Feb 7.


Importance of material evaluation prior to the construction of devices for in vitro techniques.

Rach J, Halter B, Aufderheide M.

Exp Toxicol Pathol. 2013 Nov;65(7-8):973-8. doi: 10.1016/j.etp.2013.01.011. Epub 2013 Feb 26.


Detection of the cytotoxicity of water-insoluble fraction of cigarette smoke by direct exposure to cultured cells at an air-liquid interface.

Nara H, Fukano Y, Nishino T, Aufderheide M.

Exp Toxicol Pathol. 2013 Jul;65(5):683-8. doi: 10.1016/j.etp.2012.08.004. Epub 2012 Sep 19.


Analytical in vitro approach for studying cyto- and genotoxic effects of particulate airborne material.

Aufderheide M, Scheffler S, Möhle N, Halter B, Hochrainer D.

Anal Bioanal Chem. 2011 Dec;401(10):3213-20. doi: 10.1007/s00216-011-5163-4. Epub 2011 Jun 22.


In vitro models of inhalation toxicity and disease. The report of a FRAME workshop.

BéruBé K, Aufderheide M, Breheny D, Clothier R, Combes R, Duffin R, Forbes B, Gaça M, Gray A, Hall I, Kelly M, Lethem M, Liebsch M, Merolla L, Morin JP, Seagrave J, Swartz MA, Tetley TD, Umachandran M.

Altern Lab Anim. 2009 Feb;37(1):89-141. No abstract available.


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