Format

Send to

Choose Destination
Polymers (Basel). 2018 Mar 1;10(3). pii: E254. doi: 10.3390/polym10030254.

Assessing Mucoadhesion in Polymer Gels: The Effect of Method Type and Instrument Variables.

Author information

1
Laboratory of Research and Development of Drug Delivery Systems, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringa, Maringa, Parana CEP 87020-900, Brazil. jessicabassidasilva@gmail.com.
2
Laboratory of Research and Development of Drug Delivery Systems, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringa, Maringa, Parana CEP 87020-900, Brazil. sbsferreira88@gmail.com.
3
Laboratory of Research and Development of Drug Delivery Systems, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringa, Maringa, Parana CEP 87020-900, Brazil. avreis77@gmail.com.
4
Research Centre in Topical Drug Delivery and Toxicology, Department of Pharmacy, Pharmacology and Postgraduate Medicine, University of Hertfordshire, Hatfield AL10 9AB, UK. m.cook5@herts.ac.uk.
5
Laboratory of Research and Development of Drug Delivery Systems, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringa, Maringa, Parana CEP 87020-900, Brazil. mlbruschi@uem.br.

Abstract

The process of mucoadhesion has been widely studied using a wide variety of methods, which are influenced by instrumental variables and experiment design, making the comparison between the results of different studies difficult. The aim of this work was to standardize the conditions of the detachment test and the rheological methods of mucoadhesion assessment for semisolids, and introduce a texture profile analysis (TPA) method. A factorial design was developed to suggest standard conditions for performing the detachment force method. To evaluate the method, binary polymeric systems were prepared containing poloxamer 407 and Carbopol 971P®, Carbopol 974P®, or Noveon® Polycarbophil. The mucoadhesion of systems was evaluated, and the reproducibility of these measurements investigated. This detachment force method was demonstrated to be reproduceable, and gave different adhesion when mucin disk or ex vivo oral mucosa was used. The factorial design demonstrated that all evaluated parameters had an effect on measurements of mucoadhesive force, but the same was not observed for the work of adhesion. It was suggested that the work of adhesion is a more appropriate metric for evaluating mucoadhesion. Oscillatory rheology was more capable of investigating adhesive interactions than flow rheology. TPA method was demonstrated to be reproducible and can evaluate the adhesiveness interaction parameter. This investigation demonstrates the need for standardized methods to evaluate mucoadhesion and makes suggestions for a standard study design.

KEYWORDS:

detachment force; pluronic f127; rheology; texture profile analysis; thermogelling polymers; thermoresponsive polymers

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center