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Int J Mol Sci. 2018 May 10;19(5). pii: E1424. doi: 10.3390/ijms19051424.

Hierarchical ZIF-8 toward Immobilizing Burkholderia cepacia Lipase for Application in Biodiesel Preparation.

Author information

1
Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. miaadadnan14@gmail.com.
2
Ministry of Science and Technology, Baghdad 10001, Iraq. miaadadnan14@gmail.com.
3
Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. D201577434@hust.edu.cn.
4
Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. jhwang@hust.edu.cn.
5
Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. xuli@hust.edu.cn.
6
Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. yanyunjun@mail.hust.edu.cn.

Abstract

A hierarchical mesoporous zeolitic imidazolate framework (ZIF-8) was processed based on cetyltrimethylammonium bromide (CTAB) as a morphological regulating agent and amino acid (l-histidine) as assisting template agent. Burkholderia cepacia lipase (BCL) was successfully immobilized by ZIF-8 as the carrier via an adsorption method (BCL-ZIF-8). The immobilized lipase (BCL) showed utmost activity recovery up to 1279%, a 12-fold boost in its free counterpart. BCL-ZIF-8 was used as a biocatalyst in the transesterification reaction for the production of biodiesel with 93.4% yield. There was no significant lowering of conversion yield relative to original activity for BCL-ZIF-8 when continuously reused for eight cycles. This work provides a new outlook for biotechnological importance by immobilizing lipase on the hybrid catalyst (ZIF-8) and opens the door for its uses in the industrial field.

KEYWORDS:

Burkholderia cepacia lipase (BCL); adsorption method; biodiesel production; hexahedral ZIF-8; hierarchical ZIF-8; surfactant

PMID:
29747462
PMCID:
PMC5983715
DOI:
10.3390/ijms19051424
[Indexed for MEDLINE]
Free PMC Article

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