Format

Send to

Choose Destination
Microorganisms. 2019 Apr 19;7(4). pii: E106. doi: 10.3390/microorganisms7040106.

Broad Environmental Tolerance for a Salicola Host-Phage Pair Isolated from the Cargill Solar Saltworks, Newark, CA, USA.

Author information

1
Department of Biological Sciences, California State University, Long Beach, CA 90840, USA. meghan.rodela@hotmail.com.
2
Department of Biological Sciences, California State University, Long Beach, CA 90840, USA. shereen.sabet@csulb.edu.
3
Department of Biological Sciences, California State University, Long Beach, CA 90840, USA. allie.peterson@me.com.
4
Department of Biological Sciences, California State University, Long Beach, CA 90840, USA. jesse.dillon@csulb.edu.

Abstract

Phages greatly influence the ecology and evolution of their bacterial hosts; however, compared to hosts, a relatively low number of phages, especially halophilic phages, have been studied. This study describes a comparative investigation of physicochemical tolerance between a strain of the halophilic bacterium, Salicola, isolated from the Cargill Saltworks (Newark, CA, USA) and its associated phage. The host grew in media between pH 6-8.5, had a salinity growth optimum of 20% total salts (ranging from 10%-30%) and an upper temperature growth limit of 48 °C. The host utilized 61 of 190 substrates tested using BIOLOG Phenotype MicroArrays. The CGφ29 phage, one of only four reported Salicola phages, is a DNA virus of the Siphoviridae family. Overall, the phage tolerated a broader range of environmental conditions than its host (salinity 0-30% total salts; pH 3-9; upper thermal limit 80 °C) and is the most thermotolerant halophilic phage ever reported. This study is the most comprehensive investigation to date of a Salicola host-phage pair and provides novel insights into extreme environmental tolerances among bacteriophages.

KEYWORDS:

Salicola; halophile; phage; saltern; thermotolerant

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center