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Sci Rep. 2018 Feb 5;8(1):2409. doi: 10.1038/s41598-018-20587-8.

Dispersion of the soybean root rot by Cycloneda sanguinea (Coleoptera: Coccinellidae).

Author information

1
Departamento de Defesa Fitossanitária, Universidade Federal de Santa Maria, Santa Maria, 97105-900, Brazil.
2
Departamento de Estatística, Universidade Federal de Santa Maria, Santa Maria, 97105-900, Brazil.
3
Departamento de Proteção de Plantas, Escola de Ciências Agronômicas, Universidade Estadual Paulista, 18603-970, Botucatu, Brazil.
4
Departamento de Fitotecnia, Universidade Federal de Viçosa, 36570-900, Viçosa, Minas Gerais, Brazil.
5
Departamento de Entomologia, Universidade Federal de Viçosa, Rio Paranaíba, 38810-000, Brazil.
6
Departamento de Entomologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, 36570-900, Brazil. zanuncio@ufv.br.

Abstract

The dispersion of pathogenic microorganisms consists of the transport of pathogens from their source to inoculate a new host. Agricultural and economic importance of the Soybean root rot justifies studying this disease, especially the role of insects as dispersers. The aim of this study was to evaluate the role of the ladybird beetle, Cycloneda sanguinea Linnaeus (Coleoptera: Coccinellidae) in the dispersion of pathogens that cause Soybean root rot. Three pathogen species, Macrophomina phaseolina (Tassi) (Sphaeropsidales: Botryosphaeriaceae), Fusarium incarnatum-equiseti species complex (FIESC), and F. commune (Skovgaard) O'Donnell & Nirenberg were isolated from the midgut of ladybird beetles and cultured. Macrophomina phaseolina was identified by morphology while for the other two species, DNA was sequenced. The DNA extracted was amplified in the Internal Transcriber Spacer (ITS) region, sequenced and compared to voucher sequences deposited in the GenBank. Sequences of nucleotide ITS1-5.8 S were identified in the regions of rDNA-ITS4 ribosomal DNA. This is the first report of Macrophomina phaseolina (Tassi) (Sphaeropsidales: Botryosphaeriaceae), Fusarium incarnatum-equiseti species complex (FIESC), and F. commune (Skovgaard) O'Donnell & Nirenberg, being dispersed by C. sanguinea in Brazilian soybean fields.

PMID:
29402912
PMCID:
PMC5799320
DOI:
10.1038/s41598-018-20587-8
[Indexed for MEDLINE]
Free PMC Article

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