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Int J Mol Sci. 2011;12(10):6796-809. doi: 10.3390/ijms12106796. Epub 2011 Oct 14.

Nuclear markers of Danube Sturgeons hybridization.

Author information

1
Department of Biochemistry and Molecular Biology, University of Bucharest, Splaiul Independentei, Bucharest 91-95, 050095, Romania; E-Mails: tn_andreea@yahoo.com (A.D.); georgescu_se@yahoo.com (S.E.G.).

Abstract

Acipenseriformes are composed of 25 sturgeon species and two paddlefish species distributed exclusively in the northern hemisphere. The Danube River and the Black Sea were originally inhabited by six sturgeon species but two are extinct and only four are still reproducing currently in the Lower Danube: Huso huso, Acipenser stellatus, A. gueldenstaedtii and A. ruthenus. Sturgeon species hybridize more easily than other fish and the determination of pure species or hybrid status is important for conservation and for breeding in fish farms. This survey demonstrated that morphological determination of this status is not reliable and a molecular tool, based on eight microsatellites genotypes is proposed. This method, based on three successive statistical analyses including Factorial Correspondence Analysis (FCA), Structure assignation and NewHybrids status determination, showed a high efficiency in discriminating pure species specimens from F1, F2 and two kinds of backcross individuals involving three of the four reproducing Lower Danube sturgeon species.

KEYWORDS:

hybrids; microsatellites; statistical analysis; sturgeons

PMID:
22072920
PMCID:
PMC3211011
DOI:
10.3390/ijms12106796
[Indexed for MEDLINE]
Free PMC Article

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