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Materials (Basel). 2015 Sep 10;8(9):6062-6070. doi: 10.3390/ma8095291.

In Vitro Studies of Polyhedral Oligo Silsesquioxanes: Evidence for Their Low Cytotoxicity.

Author information

1
Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, Lodz 90-236, Poland. ankuj@poczta.onet.pl.
2
Centre of Molecular and Macromolecular Sciences, Sienkiewicza 112, Lodz 90-236, Poland. kgradzin@cbmm.lodz.pl.
3
Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, Lodz 90-236, Poland. m_monika123@interia.eu.
4
Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, Lodz 90-236, Poland. aklajn@biol.uni.lodz.pl.
5
Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Strasse 6, Dresden 01069, Germany. aklajn@biol.uni.lodz.pl.
6
Centre of Molecular and Macromolecular Sciences, Sienkiewicza 112, Lodz 90-236, Poland. was@cbmm.lodz.pl.

Abstract

As scientific literature considers polyhedral oligosilsesquioxanes (POSS) as potential drug delivery systems, it is necessary to check their impact on mammalian cells. Toxicity of octaammonium chloride salt of octaaminopropyl polyhedral oligomeric silsesquioxane (oap-POSS) towards two cell lines: mouse neuroblastoma (N2a) and embryonic mouse hippocampal cells (mHippoE-18) was studied. Experiments consisted of analysis of a cell cycle, cell viability, amount of apoptotic and necrotic cells, and generation of reactive oxygen species (ROS). POSS caused a shift in the cell population from the S and M/G₂ phases to the G₀/G₁ phase. However, the changes affected less than 10% of the cell population and were not accompanied by increased cytotoxicity. POSS did not induce either apoptosis or necrosis and did not generate reactive oxygen species. A cytotoxicity profile of POSS makes it a promising starting material as drug carrier.

KEYWORDS:

MTT assay; POSS; apoptosis; cell cycle; in vitro toxicity; necrosis; reactive oxygen species; silsesquioxane

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