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J Reprod Dev. 2015;61(5):485-8. doi: 10.1262/jrd.2015-047. Epub 2015 Jun 7.

Development of a programmable piggyback syringe pump and four-times-a-day injection regimen for superovulation in non-lactating Holstein cows.

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1
United Graduate School of Veterinary Science, Gifu University, Gifu 501-1193, Japan.

Abstract

The objectives of the present study were to develop a programmable piggyback syringe pump for bovine superovulation and to evaluate the effects of a four-times-a-day injection regimen using the pump. Non-lactating Holstein cows were treated with a total of 30 armour units of porcine FSH by injection four times a day with the pump (study, n = 9) or injection twice a day manually (control, n = 9) for four consecutive days from D10 of the estrous cycle. The pump-driven program successfully induced superovulation in all cows tested. The numbers of small (3- < 5 mm in diameter) and large (≥ 10 mm in diameter) follicles were greater in the study group on D11-13 and D14, respectively. There were fewer unovulated follicles detected on D21 (7 days after estrus) in the study group than in the control group (1.2 ± 0.4 and 3.2 ± 0.6, respectively).

PMID:
26052155
PMCID:
PMC4623155
DOI:
10.1262/jrd.2015-047
[Indexed for MEDLINE]
Free PMC Article

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