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Items: 1 to 20 of 41


The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones.

Watanabe S, Sakurai T, Nakamura S, Miyoshi K, Sato M.

Int J Mol Sci. 2018 Apr 4;19(4). pii: E1075. doi: 10.3390/ijms19041075.


Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.

Sato M, Miyoshi K, Nakamura S, Ohtsuka M, Sakurai T, Watanabe S, Kawaguchi H, Tanimoto A.

Int J Mol Sci. 2017 Dec 4;18(12). pii: E2610. doi: 10.3390/ijms18122610.


Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes.

Sato M, Kosuke M, Koriyama M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K.

Theriogenology. 2018 Mar 1;108:29-38. doi: 10.1016/j.theriogenology.2017.11.030. Epub 2017 Nov 26.


Selection of human blastocysts with a high implantation potential based on timely compaction.

Mizobe Y, Ezono Y, Tokunaga M, Oya N, Iwakiri R, Yoshida N, Sato Y, Onoue N, Miyoshi K.

J Assist Reprod Genet. 2017 Aug;34(8):991-997. doi: 10.1007/s10815-017-0962-y. Epub 2017 Jun 1.


Birth of Cloned Microminipigs Derived from Somatic Cell Nuclear Transfer Embryos That Have Been Transiently Treated with Valproic Acid.

Miyoshi K, Kawaguchi H, Maeda K, Sato M, Akioka K, Noguchi M, Horiuchi M, Tanimoto A.

Cell Reprogram. 2016 Nov;18(6):390-400.


Developmental ability of embryos produced from oocytes with fragile oolemma by intracytoplasmic sperm injection.

Mizobe Y, Oya N, Iwakiri R, Yoshida N, Sato Y, Onoue N, Miyoshi K, Tokunaga M, Ezono Y.

J Assist Reprod Genet. 2016 Dec;33(12):1685-1690. Epub 2016 Sep 17.


The piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs.

Sato M, Maeda K, Koriyama M, Inada E, Saitoh I, Miura H, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K.

Int J Mol Sci. 2016 Aug 30;17(9). pii: E1424. doi: 10.3390/ijms17091424.


Effects of early cleavage patterns of human embryos on subsequent in vitro development and implantation.

Mizobe Y, Oya N, Iwakiri R, Yoshida N, Sato Y, Miyoshi K, Tokunaga M, Ezono Y.

Fertil Steril. 2016 Aug;106(2):348-353.e2. doi: 10.1016/j.fertnstert.2016.04.020. Epub 2016 Apr 30.


Preincubation with green tea polyphenol extract is beneficial for attenuating sperm injury caused by freezing-thawing in swine.

Kitaji H, Ookutsu S, Sato M, Miyoshi K.

Anim Sci J. 2015 Nov;86(11):922-8. doi: 10.1111/asj.12379. Epub 2015 Aug 7.


Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations.

Sato M, Koriyama M, Watanabe S, Ohtsuka M, Sakurai T, Inada E, Saitoh I, Nakamura S, Miyoshi K.

Int J Mol Sci. 2015 Aug 3;16(8):17838-56. doi: 10.3390/ijms160817838.


Production of Cloned Miniature Pigs Expressing High Levels of Human Apolipoprotein(a) in Plasma.

Ozawa M, Himaki T, Ookutsu S, Mizobe Y, Ogawa J, Miyoshi K, Yabuki A, Fan J, Yoshida M.

PLoS One. 2015 Jul 6;10(7):e0132155. doi: 10.1371/journal.pone.0132155. eCollection 2015.


A new rolling culture-based in vitro fertilization system capable of reducing polyspermy in porcine oocytes.

Kitaji H, Ookutsu S, Sato M, Miyoshi K.

Anim Sci J. 2015 May;86(5):494-8. doi: 10.1111/asj.12327. Epub 2014 Nov 30.


The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of the α-1,3-galactosyltransferase gene in porcine embryonic fibroblasts.

Sato M, Miyoshi K, Nagao Y, Nishi Y, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S.

Xenotransplantation. 2014 May-Jun;21(3):291-300. doi: 10.1111/xen.12089. Epub 2014 Feb 21.


Effect of postactivation treatment with latrunculin A on in vitro and in vivo development of cloned embryos derived from kidney fibroblasts of an aged Clawn miniature boar.

Himaki T, Mizobe Y, Tsuda K, Suetomo M, Yamakuchi H, Miyoshi K, Takao S, Yoshida M.

J Reprod Dev. 2012;58(4):398-403. Epub 2012 Apr 13.


Gene expression profile differences in embryos derived from prepubertal and adult Japanese Black cattle during in vitro development.

Dorji, Ohkubo Y, Miyoshi K, Yoshida M.

Reprod Fertil Dev. 2012;24(2):370-81. doi: 10.1071/RD11048.


Expression analysis of an α-1, 3-galactosyltransferase, an enzyme that creates xenotransplantation-related α-Gal epitope, in pig preimplantation embryos.

Chi H, Sato M, Yoshida M, Miyoshi K.

Anim Sci J. 2012 Jan;83(1):88-93. doi: 10.1111/j.1740-0929.2011.00964.x. Epub 2011 Oct 18.


Successful suppression of endogenous α-1,3-galactosyltransferase expression by RNA interference in pig embryos generated in vitro.

Chi H, Shinohara M, Yokomine T, Sato M, Takao S, Yoshida M, Miyoshi K.

J Reprod Dev. 2012;58(1):69-76. Epub 2011 Oct 14.


Whole-genome amplification-based GenomiPhi for multiple genomic analysis of individual early porcine embryos.

Akasaka E, Ozawa A, Mori H, Mizobe Y, Yoshida M, Miyoshi K, Sato M.

Theriogenology. 2011 May;75(8):1543-9. doi: 10.1016/j.theriogenology.2010.12.018. Epub 2011 Feb 26.


Effects of trichostatin A on in vitro development and transgene function in somatic cell nuclear transfer embryos derived from transgenic Clawn miniature pig cells.

Himaki T, Yokomine TA, Sato M, Takao S, Miyoshi K, Yoshida M.

Anim Sci J. 2010 Oct;81(5):558-63. doi: 10.1111/j.1740-0929.2010.00772.x.


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